Cell Signaling Technology

Product Pathways - Tyrosine Kinase/ Adaptors

SCF Antibody #2273

Applications Reactivity MW (kDa) Source
W H 18kDa Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

SCF antibody detects 5 ng of recombinant SCF.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to amino acids near the amino terminus of human SCF. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of recombinant SCF #9907, using SCF Antibody.

Background

Stem cell factor (SCF) is a growth factor that is essential for haemopoiesis, melanogenesis and fertility. SCF is also known as mast cell growth factor (MCGF), steel factor (SLF), or kit ligand (KL) (1-3). SCF mediates its biological effects by binding to and activating c-Kit (4). SCF induces dimerization of c-Kit followed by trans-autophosphorylation of the cytoplasmic protein tyrosine kinase domain, leading to subsequent recruitment of signaling proteins, tyrosine phosphorylation of substrates, and activation of multiple signaling pathways (5,6). SCF/c-Kit may take part in the growth control of human malignancies (7).

  1. Smith, M.A. et al. (2001) Blood Rev. 15, 191-197.
  2. Linnekin, D. (1999) Int. J. Biochem. Cell Biol. 31, 1053-1074.
  3. Huang, E. et al. (1990) Cell 63, 225-233.
  4. Jiang, X. et al. (2000) EMBO J. 19, 3192-3203.
  5. Ashman, L.K. (1999) Int. J. Biochem. Cell Biol. 31, 1037-1051.
  6. Ueda, S. et al. (2002) Blood 99, 3342-3349.
  7. Attoub, S. et al. (2002) Cancer Res. 62, 4879-4883.

Application References

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