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Myc-Tag (9B11) Mouse mAb #2276

Applications Reactivity Sensitivity Isotype
W IP IF-IC F All Transfected Only Mouse IgG2a

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key: All=All species expected

Specificity / Sensitivity

Myc-Tag (9B11) Mouse mAb detects transfected proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag fused to either the amino- or carboxy-termini of targeted proteins in transfected mammalian cells.

Source / Purification

Monoclonal antibody is produced by immunizing mice with a synthetic peptide (KLH-coupled) corresponding to residues 410-419 of human c-Myc (EQKLISEEDL).

Western Blotting

Western Blotting

Western blot analysis of cell extracts expressing carboxy-terminal Myc-tagged protein (lane 1), amino-terminal Myc-tagged protein (lane 2) or control cell extracts (lane 3), using Myc-Tag (9B11) Mouse mAb.

IP

IP

Immunoprecipitation of cell extracts alone (lane 1), extracts overexpressing carboxy-terminal Myc-tagged protein (lane 2) or amino-terminal Myc-tagged protein (lane 3), using Myc-Tag (9B11) Mouse mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of COS cells, untreated (green) or Myc transfected (blue), using Myc-Tag (9B11) Mouse mAb.


IF-IC

IF-IC

Immunocytochemical staining of cells without transfected protein (left) and cells expressing carboxy-terminal Myc-tagged protein (right), using Myc-Tag (9B11) Mouse mAb.

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.

The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems (1).

  1. Munro, S. and Pelham, H.R. (1984) EMBO J. 3, 3087-3093.

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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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