Product Pathways - Complementary Reagents

Myc-Tag (9B11) Mouse mAb #2276

Applications	Reactivity	Sensitivity	Isotype
W IP IHC-P IF-IC F	All	Transfected Only	Mouse IgG2a

Applications Key: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.

Protocols

2276:: Flow, IHC / Paraffin, Immunofluorescence, Immunoprecipitation, Western Blotting

Specificity / Sensitivity

Myc-Tag (9B11) Mouse mAb detects recombinant proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues 410-419 of human c-Myc (EQKLISEEDL).

Western Blotting

Western blot analysis of cell extracts expressing carboxy-terminal Myc-tagged protein (lane 1), amino-terminal Myc-tagged protein (lane 2) or control cell extracts (lane 3), using Myc-Tag (9B11) Mouse mAb.

IP

Immunoprecipitation of cell extracts alone (lane 1), extracts overexpressing carboxy-terminal Myc-tagged protein (lane 2) or amino-terminal Myc-tagged protein (lane 3), using Myc-Tag (9B11) Mouse mAb.

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded COS cell pellets, control (left) or transfected with a carboxy-terminal Myc tagged protein (right), using Myc-Tag (9B11) Mouse mAb.

Flow Cytometry

Flow cytometric analysis of COS cells, untreated (green) or Myc transfected (blue), using Myc-Tag (9B11) Mouse mAb.

IF-IC

Immunocytochemical staining of cells without transfected protein (left) and cells expressing carboxy-terminal Myc-tagged protein (right), using Myc-Tag (9B11) Mouse mAb.

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.

The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems (1).

Munro, S. and Pelham, H.R. (1984) EMBO J. 3, 3087-3093.

Application References

Lee, J. H. et al. (2003) The neuronal adaptor protein X11alpha reduces Abeta levels in the brains of Alzheimer's APPswe Tg2576 transgenic mice. J. Biol. Chem. 278, 47025-47029. Applications: IC-ABC Western Blotting
Giles, N. et al. (2003) 14-3-3 acts as an intramolecular bridge to regulate cdc25B localization and activity. J. Biol. Chem. 278, 28580-28587. Applications: IP Western Blotting
Miyazaki, K. et al. (2004) NEDL1, a novel ubiquitin-protein isopeptide ligase for dishevelled-1, targets mutant superoxide dismutase-1. J. Biol. Chem. 279, 11327-11335. Applications: IP Western Blotting
Kim, D. W. et al. (2003) RET/PTC (rearranged in transformation/papillary thyroid carcinomas) tyrosine kinase phosphorylates and activates phosphoinositide-dependent kinase 1 (PDK1): an alternative phosphatidylinositol 3-kinase-independent pathway to activate PDK1. Mol. Endocrinol. 17 (7), 1382-1394. Applications: IP Western Blotting
Cormier, J.H. et al. (2009) Mol Cell 34, 627-33. Applications: IP
Boggiano, J.C. et al. (2011) Dev Cell 21, 888-95. Applications: Western Blotting
Jeong, H. et al. (2011) Nat Med 18, 159-65. Applications: IP
Zheng, Y. et al. (2012) J Biol Chem 287, 148-58. Applications: IF-IC (In Cells) Western Blotting

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.