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Myc-Tag (9B11) Mouse mAb #2276
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| W IP IF-IC F | All | Transfected Only | Mouse IgG2a |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key: All=All species expected
Specificity / Sensitivity
Myc-Tag (9B11) Mouse mAb detects transfected proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag fused to either the amino- or carboxy-termini of targeted proteins in transfected mammalian cells.
Source / Purification
Monoclonal antibody is produced by immunizing mice with a synthetic peptide (KLH-coupled) corresponding to residues 410-419 of human c-Myc (EQKLISEEDL).
Western Blotting
Western blot analysis of cell extracts expressing carboxy-terminal Myc-tagged protein (lane 1), amino-terminal Myc-tagged protein (lane 2) or control cell extracts (lane 3), using Myc-Tag (9B11) Mouse mAb.
IP
Immunoprecipitation of cell extracts alone (lane 1), extracts overexpressing carboxy-terminal Myc-tagged protein (lane 2) or amino-terminal Myc-tagged protein (lane 3), using Myc-Tag (9B11) Mouse mAb.
Flow Cytometry
Flow cytometric analysis of COS cells, untreated (green) or Myc transfected (blue), using Myc-Tag (9B11) Mouse mAb.
IF-IC
Immunocytochemical staining of cells without transfected protein (left) and cells expressing carboxy-terminal Myc-tagged protein (right), using Myc-Tag (9B11) Mouse mAb.
Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.
The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems (1).
Application References
- Ho, C. S. et al. (2002) Synergistic effects of Munc18a and X11 proteins on amyloid precursor protein metabolism. J. Biol. Chem. 277, 27021-27028. This article references the use of Myc-Tag (9B11) Mouse mAb in the following applications: IP
- Lee, J. H. et al. (2003) The neuronal adaptor protein X11alpha reduces Abeta levels in the brains of Alzheimer's APPswe Tg2576 transgenic mice. J. Biol. Chem. 278, 47025-47029. This article references the use of Myc-Tag (9B11) Mouse mAb in the following applications: IC-ABC Western Blotting
- Giles, N. et al. (2003) 14-3-3 acts as an intramolecular bridge to regulate cdc25B localization and activity. J. Biol. Chem. 278, 28580-28587. This article references the use of Myc-Tag (9B11) Mouse mAb in the following applications: IP Western Blotting
- Miyazaki, K. et al. (2004) NEDL1, a novel ubiquitin-protein isopeptide ligase for dishevelled-1, targets mutant superoxide dismutase-1. J. Biol. Chem. 279, 11327-11335. This article references the use of Myc-Tag (9B11) Mouse mAb in the following applications: IP Western Blotting
- Kim, D. W. et al. (2003) RET/PTC (rearranged in transformation/papillary thyroid carcinomas) tyrosine kinase phosphorylates and activates phosphoinositide-dependent kinase 1 (PDK1): an alternative phosphatidylinositol 3-kinase-independent pathway to activate PDK1. Mol. Endocrinol. 17 (7), 1382-1394. This article references the use of Myc-Tag (9B11) Mouse mAb in the following applications: IP Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
