Myc-Tag (71D10) Rabbit mAb #2278
|2278S||100 µl (10 western blots)||---||In Stock||---|
|2278P||40 µl (4 western blots)||---||In Stock||---|
|2278||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Transfected Only||Rabbit IgG|
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Specificity / Sensitivity
Myc-Tag (71D10) Rabbit mAb detects recombinant proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues 410-419 of human c-Myc (EQKLISEEDL).
Western blot analysis of extracts from untransfected control cells (-) and transfected cells overexpressing Myc-Bcl-2 (+), using Bcl-2 Antibody #2872 (left) and Myc-Tag (71D10) Rabbit mAb (right).
Flow cytometric analysis of COS cells, untreated (blue) or Myc transfected (green), using Myc-Tag (71D10) Rabbit mAb.
Immunocytochemical staining of cells expressing Myc-Bcl-2 protein (left) or cells without transfected protein (right), using Myc-tag (71D10) Rabbit mAb.
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.
The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems (1).
- Munro, S. and Pelham, H.R. (1984) EMBO J. 3, 3087-3093.
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
This antibody is developed, validated, and produced by CST using in part technology under license (granting certain rights including those under U.S. Patents No. 5,675,063 and 7,429,487) from Epitomics, Inc.