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Myc-Tag (71D10) Rabbit mAb #2278
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| W IP IF-IC F | All | Transfected Only | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key: All=All species expected
Specificity / Sensitivity
Myc-Tag (71D10) Rabbit mAb detects transfected proteins containing the Myc epitope tag.
Source / Purification
Monoclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues 410-419 of human c-Myc (EQKLISEEDL).
Western Blotting
Western blot analysis of extracts from untransfected control cells (-) and transfected cells overexpressing Myc-Bcl-2 (+), using Bcl-2 Antibody #2872 (left) and Myc-Tag (71D10) Rabbit mAb (right).
Flow Cytometry
Flow cytometric analysis of COS cells, untreated (blue) or Myc transfected (green), using Myc-Tag (71D10) Rabbit mAb.
IF-IC
Immunocytochemical staining of cells expressing Myc-Bcl-2 protein (left) or cells without transfected protein (right), using Myc-tag (71D10) Rabbit mAb.
Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.
The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems (1).
- Munro, S. and Pelham, H.R. (1984) EMBO J. 3, 3087-3093.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
