Product Pathways - Glucose Metabolism
SCD1 (R347) Antibody #2283
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IF-IC | M (H) | Endogenous | 37 | Rabbit |
Applications Key:
W=Western Blotting
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
M=Mouse
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
SCD1 (R347) Antibody detects endogenous levels of total SCD1 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) derived from the sequence around R347 of human SCD1. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of 3T3-L1 cell lysates, using SCD1 (R347) Antibody.
IF-IC
Confocal immunofluorescent analysis of 3T3-L1 cells labeled with SCD1 (R347) Antibody (green) showing cytoplasmic localization in differentiated cells. Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Background
Stearoyl-CoA desaturase 1 (SCD1) is a key lipogenic enzyme found in the endoplasmic reticulum that catalyzes the conversion of palmitoyl?CoA and stearoyl?CoA to palmitoleoyl?CoA (16:1) and oleoyl?CoA (18:1) (1-3). Palmitoleate and oleate are the major components of triglycerides, membrane phospholipids and cholesterol esters (1). SCD1-knockout mice show improved insulin sensitivity and reduced body fat (1). Disruption of SCD1 in mouse brown adipose tissue strengthens insulin signaling and results in increased translocation of Glut4 to plasma membrane and enhanced uptake of glucose (4). Furthermore, SCD1 is essential for the onset of diet-induced body weight gain (1) and insulin resistance in the liver (5).
- Ntambi, J.M. et al. (2002) Proc. Natl. Acad. Sci. USA 99, 11482-114866.
- Kato, H. et al. (2006) J. Cell. Sci. 119, 2342-2353.
- Ozols, J. (1997) Mol. Biol. Cell 8, 2281-2290.
- Rahman, S.M. et al. (2005) Am. J Physiol. Endocrinol. Metab. 288, E381-387.
- Gutierrez-Juarez, R. et al. (2006) J. Clin. Invest. 116, 1686-1695.
Application References
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
