Product Pathways - Protein Folding/Stability
Cathepsin D Antibody #2284
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IHC-P IF-IC F | H Mk | Endogenous | 46,43,28 | Rabbit |
Applications Key:
W=Western Blotting
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Cathepsin D Antibody detects endogenous levels of preprocathepsin D, procathepsin D and the heavy chain subunit of mature cathepsin D.
Source / Purification
Polyclonal antibodies are prepared by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues with the heavy chain subunit of human Cathepsin D. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from PANC1, SNB19 and MCF-7 cell lines, using Cathepsin D Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Cathepsin D Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human Non-Hodgkin's lymphoma, using Cathepsin D Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human papillary carcinoma, using Cathepsin D Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human serous adenocarcinoma, using Cathepsin D Antibody.
Flow Cytometry
Flow cytometric analysis of MCF-7 cells, using Cathepsin D Antibody (blue) compared to a nonspecific negative control antibody (red).
IF-IC
Immunofluorescent analysis of ACHN cells showing lysosomal localization, using Cathepsin D Antibody.
Background
Cathepsin D is a ubiquitously expressed lysosomal aspartyl protease involved in the normal degradation of proteins (1). It is synthesized as an inactive 43 kDa preprocathepsin D that is cleaved and glycosylated to form a 46 kDa procathepsin D and then further cleaved to produce 28 kDa and 15 kDa subunits (heavy and light chains, respectively) (2). Cathepsin D may also be secreted into the cytosol during apoptosis and contribute to cleavage of substrates implicated in the apoptotic pathway (3). Numerous studies have suggested that cathepsin D plays a role in neuronal degradation and malignant transformation, particularily in breast cancer (4-9).
- Faust, P.L. et al. (1985) Proc. Natl. Acad. Sci. USA 82, 4910-4914.
- Erickson, A.H. et al. (1981) J. Biol .Chem. 256, 11224-11231.
- Liaudet-Coopman, E. et al. (2006) Cancer Lett. 237, 167-179.
- Berchem, G. et al. (2002) Oncogene 21, 5951-5955.
- Nomura, T. and Katunuma, N. (2005) J. Med. Invest. 52, 1-9.
- Garcia, M. et al. (1996) Stem Cells 14, 642-560.
- Nogami, M. et al. (2000) Histochem. J. 32, 505-508.
- Nakanishi, H. (2003) Ageing Res. Rev. 2, 367-381.
- Callahan, L.M. et al. Neurobiol. Aging 19, S99-105.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
