Product Pathways - Protein Stability
Cathepsin D Antibody #2284
PhosphoSitePlus® protein, site, and accession data: CTSD
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IHC-P F | H Mk | Endogenous | 46,43,28 | Rabbit |
Applications Key:
W=Western Blotting
IHC-P=Immunohistochemistry (Paraffin)
F=Flow Cytometry
Reactivity Key:
H=Human
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 2284:
- Flow, IHC / Paraffin, Western Blotting
Specificity / Sensitivity
Cathepsin D Antibody detects endogenous levels of preprocathepsin D, procathepsin D and the heavy chain subunit of mature cathepsin D.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues with the heavy chain subunit of human Cathepsin D. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from PANC1, SNB19 and MCF-7 cell lines using Cathepsin D Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Cathepsin D Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human Non-Hodgkin's lymphoma using Cathepsin D Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human papillary carcinoma using Cathepsin D Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human serous adenocarcinoma using Cathepsin D Antibody.
Flow Cytometry
Flow cytometric analysis of MCF-7 cells using Cathepsin D Antibody (blue) compared to a nonspecific negative control antibody (red).
Background
Cathepsin D is a ubiquitously expressed lysosomal aspartyl protease involved in the normal degradation of proteins (1). It is synthesized as an inactive 43 kDa preprocathepsin D that is cleaved and glycosylated to form a 46 kDa procathepsin D and then further cleaved to produce 28 kDa and 15 kDa subunits (heavy and light chains, respectively) (2). Cathepsin D may also be secreted into the cytosol during apoptosis and contribute to cleavage of substrates implicated in the apoptotic pathway (3). Numerous studies have suggested that cathepsin D plays a role in neuronal degradation and malignant transformation, particularily in breast cancer (4-9).
- Faust, P.L. et al. (1985) Proc. Natl. Acad. Sci. USA 82, 4910-4914.
- Erickson, A.H. et al. (1981) J. Biol .Chem. 256, 11224-11231.
- Liaudet-Coopman, E. et al. (2006) Cancer Lett. 237, 167-179.
- Berchem, G. et al. (2002) Oncogene 21, 5951-5955.
- Nomura, T. and Katunuma, N. (2005) J. Med. Invest. 52, 1-9.
- Garcia, M. et al. (1996) Stem Cells 14, 642-560.
- Nogami, M. et al. (2000) Histochem. J. 32, 505-508.
- Nakanishi, H. (2003) Ageing Res. Rev. 2, 367-381.
- Callahan, L.M. et al. Neurobiol. Aging 19, S99-105.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 8114 SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)
- 8112 SignalStain® Antibody Diluent
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
