Product Pathways - Lymphocyte Signaling
Thioredoxin 1 Antibody (Human Specific) #2285
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H | Endogenous | 12 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Thioredoxin 1 Antibody (human-specific) detects endogenous levels of total human thioredoxin 1 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) derived from the sequence of human thioredoxin 1. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from various cell lines, using Thioredoxin 1 Antibody (Human Specific).
Background
Thioredoxin is a small redox protein found in many eukaryotes and prokaryotes. A pair of cysteines within a highly conserved, active site sequence can be oxidized to form a disulfide bond which is then reduced by thioredoxin reductase (1). Multiple forms of thioredoxin have been identified, including cytosolic thioredoxin 1 (Trx1) and mitochondrial thioredoxin 2 (Trx2). Thioredoxin participates in many cellular processes including redox signaling, response to oxidative stress and protein reduction (1). A potential role of thioredoxin in human disorders such as cancer, aging and heart disease is currently under investigation (2). Thioredoxin can play a key role in cancer progression, as it acts as a negative regulator of the proapoptotic kinase ASK1 (3) Changes in thioredoxin expression have been associated with meningococcal septic shock and acute lung injury (4,5).
- Watson, W.H. et al. (2004) Toxicol. Sci. 78, 3-14.
- Burke-Gaffney, A. et al. (2005) Trends Pharmacol. Sci. 26, 398-404.
- Saitoh, M. et al. (1998) EMBO J. 17, 2596-2606.
- Callister, M.E. et al. (2007) Intensive Care Med. 33, 364-367.
- Callister, M.E. et al. (2006) Thorax 61, 521-527.
Application References
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
