Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

Thioredoxin 1 Antibody (Human Specific) #2285

Applications Reactivity Sensitivity MW (kDa) Source
W H Endogenous 12 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Thioredoxin 1 Antibody (human-specific) detects endogenous levels of total human thioredoxin 1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human thioredoxin 1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines, using Thioredoxin 1 Antibody (Human Specific).

Background

Thioredoxin is a small redox protein found in many eukaryotes and prokaryotes. A pair of cysteines within a highly conserved, active site sequence can be oxidized to form a disulfide bond which is then reduced by thioredoxin reductase (1). Multiple forms of thioredoxin have been identified, including cytosolic thioredoxin 1 (Trx1) and mitochondrial thioredoxin 2 (Trx2). Thioredoxin participates in many cellular processes including redox signaling, response to oxidative stress and protein reduction (1). A potential role of thioredoxin in human disorders such as cancer, aging and heart disease is currently under investigation (2). Thioredoxin can play a key role in cancer progression, as it acts as a negative regulator of the proapoptotic kinase ASK1 (3) Changes in thioredoxin expression have been associated with meningococcal septic shock and acute lung injury (4,5).

  1. Watson, W.H. et al. (2004) Toxicol. Sci. 78, 3-14.
  2. Burke-Gaffney, A. et al. (2005) Trends Pharmacol. Sci. 26, 398-404.
  3. Saitoh, M. et al. (1998) EMBO J. 17, 2596-2606.
  4. Callister, M.E. et al. (2007) Intensive Care Med. 33, 364-367.
  5. Callister, M.E. et al. (2006) Thorax 61, 521-527.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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