Cell Signaling Technology

Product Pathways - Development

Shh Antibody #2287

Applications Reactivity Sensitivity MW (kDa) Source
W IP M (H) (Dg) Transfected Only 19, 42, 45 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  Dg=Dog
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Shh antibody detects both the precursor and mature forms of transfected Shh.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His35 of human Shh. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of total cell lysates from 293 cells and 293 cells transiently transfected with mouse Shh construct, using Shh antibody.

Background

Hedgehog proteins (Hh) are secreted signaling proteins that play many roles during animal development. Aberrant Hh signaling activity can be associated with numerous birth defects and uncontrolled Hh pathway activation is linked to the development of several types of cancers (1-2). The three identified vertebrate Hh genes are Sonic (Shh), Indian (Ihh) and Desert (Dhh), all of which have distinct as well as overlapping roles (3-5). Hh proteins are synthesized as 45 kDa precursors that undergo auto-cleavage to generate a 19 kDa amino-terminal peptide (Hh-N) and a carboxy-terminal peptide (Hh-C). The amino-terminal peptide becomes covalently attached to a cholesterol molecule at its carboxy terminus and acetylated at its amino terminus. This doubly modified Hh-N peptide is released from cells and responsible for all known Hedgehog signaling activity (6).

  1. Ingham, P.W. and McMahon, A.P. (2001) Genes Dev. 15, 3059-3087.
  2. McMahon, A.P. et al. (2003) Curr. Top Dev. Biol. 53, 1-114.
  3. Zhang, X.M. et al. (2001) Cell 106, 781-792.
  4. Adolphe, C. et al. (2004) Development 131, 5009-5019.
  5. Pathi, S. et al. (2001) Mech. Dev. 106, 107-117.
  6. Bijlsma, M.F. et al. (2004) Bioessays 26, 387-394.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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