Cell Signaling Technology

Product Pathways - Phosphatases

PP5 Antibody #2289

Applications Reactivity Sensitivity MW (kDa) Source
W F H M R Mk Endogenous 58 Rabbit

Applications Key:  W=Western Blotting  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

PP5 Antibody detects endogenous levels of total PP5 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal sequence of human PP5. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293, NIH/3T3 and PC12 cells, using PP5 Antibody.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells, using PP5 Antibody (blue) compared to a nonspecific negative control antibody (red).

Background

Protein phosphatase 5 (PP5) is a member of the serine/threonine phosphatase family that also includes PP1, PP2A and PP2B. PP5 uniquely contains an amino-terminal regulatory domain with three tetratricopeptide repeat (TPR) motifs and a carboxy-terminal catalytic domain (1-3). Through the TPR domain, PP5 interacts with a number of proteins and has been reported to be involved in diverse signal transduction pathways, including glucocorticoid receptor signaling, p53-mediated growth arrest, oxidative stress-mediated apoptosis and the response to ionizing radiation-induced DNA damage (4-8).

  1. Chen, M.X. and Cohen, P.T. (1997) FEBS Lett. 400, 136-140.
  2. Andreeva, A.V. and Kutuzov, M.A. (1999) Cell. Signal. 11, 555-562.
  3. Chinkers, M. Trends Endocrinol. Metab. 12, 28-32.
  4. Zuo, Z. et al. (1998) J. Biol. Chem. 273, 12250-12258.
  5. Morita, K. et al. (2001) EMBO J. 20, 6028-6036.
  6. Silverstein, A.M. et al. (1997) J. Biol. Chem. 272, 16224-16230.
  7. Ali, A. et al. (2004) Genes Dev. 18, 249-254.
  8. Wechsler, T. et al. (2004) Proc. Natl. Acad. Sci. USA 101, 1247-1252.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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