Cell Signaling Technology

Product Pathways - Phosphatases

PP2A B Subunit (100C1) Rabbit mAb #2290

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IHC-P H M R Mk Dm (C) (B) (Pg) Endogenous 52 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  C=Chicken  Dm=D. melanogaster  B=Bovine  Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

PP2A B Subunit (100C1) Rabbit mAb detects endogenous levels of the PR55 PP2A B subunit (a isoform). The antibody may also recognize the b, g, and d isoforms of the PR55 PP2A B subunit. This antibody does not cross-react with the B-prime (PR61), B-prime-prime or B-prime-prime-prime families of PP2A B subunits.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human PP2A B subunit.

Western Blotting

Western Blotting

Western blot analysis of cell lysates from HeLa, NIH/3T3, C6 and COS cells, using PP2A B Subunit (100C1) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using PP2A B Subunit (100C1) Rabbit mAb.

Background

Protein phosphatase type 2A (PP2A) is an essential protein serine/threonine phosphatase that is conserved in all eukaryotes. PP2A is a key enzyme within various signal transduction pathways as it regulates fundamental cellular activities such as DNA replication, transcription, translation, metabolism, cell cycle progression, cell division, apoptosis and development (1-3). The core enzyme consists of catalytic C and regulatory A (or PR65) subunits, with each subunit represented by α and β isoforms (1). Additional regulatory subunits belong to four different families of unrelated proteins. Both the B (or PR55) and B' regulatory protein families contain α, β, γ and δ isoforms, with the B' family also including an ε protein. B'' family proteins include PR72, PR130, PR59 and PR48 isoforms, while striatin (PR110) and SG2NA (PR93) are both members of the B''' regulatory protein family. These B subunits competitively bind to a shared binding site on the core A subunit (1). This variable array of holoenzyme components, particularly regulatory B subunits, allows PP2A to act in a diverse set of functions. PP2A function is regulated by expression, localization, holoenzyme composition and post-translational modification. Phosphorylation of PP2A at Tyr307 by Src occurs in response to EGF or insulin and results in a substantial reduction of PP2A activity (4). Reversible methylation on the carboxyl group of Leu309 of PP2A has been observed (5,6). Methylation alters the conformation of PP2A, as well as its localization and association with B regulatory subunits (6-8).

  1. Janssens, V. and Goris, J. (2001) Biochem. J. 353, 417-439.
  2. Zolnierowicz, S. (2000) Biochem. Pharmacol. 60, 1225-1235.
  3. Milward, T.A. et al. (1999) Trends Biochem. Sci. 24, 186-191.
  4. Chen, J. et al. (1992) Science 257, 1261-1264.
  5. Turowski, P. et al. (1995) J. Cell. Biol. 129, 397-410.
  6. Lee, J. et al. (1996) Proc. Natl. Acad. Sci. USA 93, 6043-6047.
  7. Tolstykh, T. et al. (2000) EMBO J. 19, 5682-5691.
  8. Yu, X.X. et al. (2001) Mol. Biol. Cell 12, 185-199.

Application References

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Companion Products

Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.

For Research Use Only. Not For Use In Diagnostic Procedures.

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