Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

IQGAP1 Antibody #2293

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Hm Mk Endogenous 195 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Hm=Hamster  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

IQGAP1 Antibody detects endogenous levels of total IQGAP1 protein. The antibody may cross-react with a 35kDa protein of unknown origin.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human IQGAP1.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types, using IQGAP1 Antibody.

Background

IQGAPs are scaffolding proteins involved in mediating cytoskeletal function. They contain multiple protein interaction domains and bind to a growing number of molecules including actin, myosin light chain, calmodulin, E-cadherin, and β-catenin (reviewed in 1). Through their GAP-related domains, they bind the small GTPases Rac1 and cdc42. IQGAPs lack GAP activity, however, and regulate small GTPases by stabilizing their GTP-bound (active) forms (2,3). Studies have shown that the function and distribution of the IQGAP proteins widely vary. IQGAP1 is ubiquitously expressed and has been found to interact with APC (4) and the CLIP170 complex (5) in response to small GTPases, promoting cell polarization and migration. Studies have suggested that IQGAP1 could play a part in the invasiveness of some cancers (6-8). IQGAP2, which is about 60% identical to IQGAP1, is expressed primarily in liver (3), but lower levels have been detected in the prostate, kidney, thyroid, stomach, and testis (9,10). It has been shown that IQGAP2 displays tumor suppressor properties (11). Less is known about the function of IQGAP3, but this protein is present in the lung, brain, small intestine, and testis (9) and is only expressed in proliferating cells (12), suggesting a role in cell growth and division.

  1. Briggs, M.W. and Sacks, D.B. (2003) EMBO Rep. 4, 571-574.
  2. Ho, Y.D. et al. (1999) J. Biol. Chem. 274, 464-470.
  3. Brill, S. et al. (1996) Mol. Cell Biol. 16, 4869-4878.
  4. Watanabe, T. et al. (2004) Dev. Cell 7, 871-883.
  5. Fukata, M. et al. (2002) Cell 109, 873-885.
  6. Chew, C.S. et al. (2005) Am J Physiol Gastrointest Liver Physiol 288, G376-87.
  7. Jin, S.H. et al. (2008) Int J Cancer 122, 1040-6.
  8. Liu, Z. et al. (2010) Clin Cancer Res 16, 6009-18.
  9. Wang, S. et al. (2007) J Cell Sci 120, 567-77.
  10. Schmidt, V.A. et al. (2003) Blood 101, 3021-8.
  11. Jin, S.H. et al. (2008) Int J Cancer 122, 1040-6.
  12. Nojima, H. et al. (2008) Nat Cell Biol 10, 971-8.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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