Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

GM130 Antibody #2296

Applications Reactivity Sensitivity MW (kDa) Source
W IP H (Mk) Endogenous 130 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

GM130 Antibody detects endogenous levels of total GM130 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal sequence of human GM130.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, ZR-75 and MCF-7 cells, using GM130 Antibody.

Background

The Golgi apparatus functions in the modification, organization, and transport of proteins and membranes targeted to other parts of the cell, such as the plasma membrane, lysosomes, and endosomes. This regulated transport is important for appropriate protein localization, secretion, and signal transduction. Members of the Golgin family of proteins, including GM130, Giantin, p115, and GRASP65, are defined by their presence in the Golgi matrix and by their long coiled coil domains. Golgin function, which is regulated in part by small GTPases of the Rab and Arl families, includes establishing and maintaining Golgi structure and transport (reviewed in 1). The Golgi cisternae are stacked and linked laterally to form a ribbon. GRASP65 and GM130 are required for membrane fusion events that mediate ribbon formation during Golgi assembly. These lateral fusion events allow for uniform distribution of Golgi enzymes (2). GM130 and Giantin interact with the transport factor p115 to facilitate endoplasmic reticulum (ER)-Golgi transport (3). GM130 is also involved in the transport of the Ether-a-go-go-Related (hERG) potassium ion channel. Inappropriate hERG localization may be an underlying cause in Long QT syndrome, a hereditary and potentially fatal cardiac arrhythmia (4). Further, GM130 was implicated in signal transduction regulating invasion, migration, and cell polarization via its interaction with and activation of serine/threonine kinases YSK1 and Mst4 (5).

  1. Barr, F.A. and Short, B. (2003) Curr. Opin. Cell Biol. 15, 405-413.
  2. Puthenveedu, M.A. et al. (2006) Nat. Cell Biol. 8, 238-248.
  3. Alvarez, C. et al. (2001) J. Biol. Chem. 276, 2693-2700.
  4. Roti, E.C. et al. (2002) J. Biol. Chem. 277, 47779-47785.
  5. Preisinger, C. et al. (2004) J. Cell Biol. 164, 1009-1020.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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