Cell Signaling Technology

Product Pathways - Translational Control

4E-T Antibody #2297

Applications Reactivity Sensitivity MW (kDa) Source
W IP H Endogenous 140 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

4E-T Antibody detects endogenous levels of total 4E-T protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human 4E-T. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines, using 4E-T Antibody.

Background

During translation, eIF4E binds to the 5' cap structure of mRNA and helps mRNA bind to the ribosome. There are several factors that associate with eIF4E. One of these factors is eIF4E-Transporter (4E-T) (1). 4E-T has an eIF4E binding site, a nuclear localization signal and two nuclear export signals (1). Studies demonstrate that 4E-T functions as a nucleocytoplasmic shuttling protein mediating the import of eIF4E into the nucleus (1). Recent findings indicate that 4E-T, along with eIF4E, is concentrated in the processing bodies (P-bodies) in the cytoplasm (2). Furthermore, 4E-T decreases mRNA stability (2).

  1. Dostie, J. et al. (2000) EMBO J 19, 3142-56.
  2. Ferraiuolo, M.A. et al. (2005) J. Cell Biol. 170, 913-924.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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