Product Pathways - Glucose Metabolism
Glut4 Antibody #2299
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M R | Endogenous | 45 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Glut4 Antibody detects endogenous levels of total Glut4 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) derived from a sequence of human Glut4 (around Q6). Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from RD and NIH/3T3-L1 cells, using Glut4 Antibody.
Background
A group of related glucose transporters (Glut1-5 and 7) mediate the facilitated diffusion of glucose in nonepithelial mammalian tissues. Within insulin-responsive tissues such as muscle and fat, Glut1 contributes to basal glucose uptake while Glut4 is responsible for insulin-stimulated glucose transport (1-3). Glut4 is a 12-transmembrane domain protein that facilitates glucose transport in the direction of the glucose gradient. This transporter localizes to intracellular organelles (endosomes) in unstimulated cells and translocates to the cell surface following insulin stimulation (1,2,4). Translocation of Glut4 is dependent on Akt, which may act by phosphorylating AS160, a RabGAP protein involved in membrane trafficking (5).
- Marette, A. et al. (1992) Am. J. Physiol. 263, C443-C452.
- Slot, J.W. et al. (1991) Proc. Natl. Acad. Sci. USA 88, 7815-7819.
- James, D.E. et al. (1989) Nature 338, 83-87.
- Barrett, M.P. et al. (1999) Curr. Opin. Cell Biol. 11, 496-502.
- Zeigerer, A. et al. (2004) Mol. Biol. Cell 15, 4406-4415.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
