Product Pathways - Neuroscience
DARPP-32 Antibody #2302
|2302S||100 µl (10 western blots)||---||In Stock||---|
|2302||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat||Endogenous||32||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Specificity / Sensitivity
DARPP-32 Antibody detects endogenous levels of total DARPP-32 independent of phosphorylation. This antibody may also detect PP1.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr34 of human DARPP-32. Antibodies are purified by protein A and peptide affinity chromatography.
DARPP-32 (dopamine and cyclic AMP-regulated phosphoprotein, relative molecular mass 32,000) is a cytosolic protein highly enriched in medium-sized spiny neurons of the neostriatum (1). It is a bifunctional signaling molecule that controls serine/threonine kinase and serine/threonine phosphatase activity (2). Dopamine stimulates phosphorylation of DARPP-32 through D1 receptors and activation of PKA. PKA phosphorylation of DARPP-32 at Thr34 converts it into an inhibitor of protein phosphatase 1 (1). DARPP-32 is converted into an inhibitor of PKA when phosphorylated at Thr75 by cyclin-dependent kinase 5 (CDK5) (2). Mice containing a targeted deletion of the DARPP-32 gene exhibit an altered biochemical, electrophysiological, and behavioral phenotype (3).
- Partida, G. J. et al. (2004) J Comp Neurol 480 (3), 251-263. Applications: Western Blotting.
- Gu, L. et al. (2009) PLoS One 4, e6220. Applications: Western Blotting.
- Danielli, B. et al. (2010) J Neurochem 112, 531-41. Applications: Western Blotting.
- Scheggi, S. et al. (2007) J Neurochem 103, 1168-83. Applications: Western Blotting.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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