Product Pathways - MAPK Signaling
JNK3 (55A8) Rabbit mAb #2305
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | H M R | Endogenous | 54 | Rabbit IgG |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
JNK3 (55A8) Rabbit Monoclonal Antibody detects endogenous levels of total JNK3 protein.
Source / Purification
Monoclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) derived from human JNK3.
Western Blotting
Western blot analysis of recombinant His-tagged JNK kinases (left), or brain lysates from wild type (WT) and specific JNK knockout (-/-) mice (right), using JNK3 (55A8) Rabbit mAb (Image provided by Drs Gerardo Morfini, YiMei You and Scott Brady, University of Illinois at Chicago).
Western Blotting
Western blot analysis of extracts from human cerebellum, mouse brain and rat brain, using JNK3 (55A8) Rabbit mAb.
Background
The stress-activated protein kinase/Jun-amino-terminal kinase (SAPK/JNK) is potently and preferentially activated by a variety of environmental stresses, including UV and gamma radiation, ceramides, inflammatory cytokines and in some instances, by growth factors and GPCR agonists (1-6). As with the other MAPKs, the core signaling unit is composed of a MAPKKK, typically MEKK1-4, or by one of the mixed lineage kinases (MLKs), which phosphorylate and activate MKK4-7, which then phosphorylate and activate the SAPK/JNK kinase (2). Stress signals are delivered to this cascade by small GTPases of the Rho family (Rac, Rho, cdc42) (3). Both Rac1 and cdc42 mediate the stimulation of MEKKs and MLKs (3). Alternatively, MKK4-7 can be activated by a pathway independent of small GTPases via stimulation of a member of the germinal center kinase (GCK) family (4). There are three SAPK/JNK genes with further diversification resulting from alternative splicing (3). Active SAPK/JNK dimers can translocate to the nucleus to regulate transcription through its effects on c-Jun, ATF-2 and other transcription factors (3,5).
- Davis, R.J. (1999) Biochem. Soc. Symp. 64, 1-12.
- Ichijo, H. (1999) Oncogene 18, 6087-6093.
- Kyriakis, J.M. and Avruch, J. (2001) Physiol. Rev. 81, 807-869.
- Kyriakis, J.M. (1999) J. Biol. Chem. 274, 5259-5262.
- Leppa, S. and Bohmann, D. (1999) Oncogene 18, 6158-6162.
- Whitmarsh, A.J. and Davis, R.J. (1998) Trends Biochem. Sci. 23, 481-485.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
