Product Pathways - MAPK Signaling
p38δ MAP Kinase (10A8) Rabbit mAb #2308
| Applications | Reactivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|
| W IP | H R | 40 | Rabbit | IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
R=Rat
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
p38δ MAP Kinase (10A8) Rabbit mAb detects endogenous levels of total p38δ MAP kinase protein. This antibody does not cross-react with other isoforms of p38 MAP kinase.
Source / Purification
Monoclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues around the carboxy terminus of p38δ MAP kinase.
Western Blotting
Western blot analysis of purified recombinant full-length p38 MAP kinase GST fusion proteins, using p38 MAP kinase pan antibody (upper), or p38δ MAP Kinase (10A8) Rabbit mAb (lower).
Western Blotting
Western blot analysis of extracts from 293, NBT-II and PC12 cells, using p38δ MAP Kinase (10A8) Rabbit mAb.
Background
p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase which participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAP kinase, p38α, β, γ (also known as ERK6 or SAPK3) and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAP kinase is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharides (LPS), UV light and growth factors (1-5). MKK3, MKK6 and SEK activate p38 MAP kinase by phosphorylation at Thr180 and Tyr182. Activated p38 MAP kinase has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6) and MEF2 (5-8).
- Rouse, J. et al. (1994) Cell 78, 1027-1037.
- Han, J. et al. (1994) Science 265, 808-811.
- Lee, J.C. et al. (1994) Nature 372, 739-746.
- Freshney, N.W. et al. (1994) Cell 78, 1039-1049.
- Raingeaud, J. et al. (1995) J. Biol. Chem. 270, 7420-7426.
- Zervos, A.S. et al. (1995) Proc. Natl. Acad. Sci. USA 92, 10531-10534.
- Zhao, M. et al. (1999) Mol. Cell. Biol. 19, 21-30.
- Yang, S.H. et al. (1999) Mol. Cell. Biol. 19, 4028-4038.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 9212 p38 MAP Kinase Antibody
- 9217 p38α MAP Kinase (5F11) Mouse mAb
- 9214 p38δ MAP Kinase Antibody
- 2371 p38α MAP Kinase (7D6) Rabbit mAb
- 9228 p38α MAP Kinase (L53F8) Mouse mAb
- 9218 p38α MAP Kinase Antibody
- 2307 p38γ MAP Kinase Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 4631 Phospho-p38 MAPK (Thr180/Tyr182)(12F8) Rabbit mAb (IHC Preferred)
- 9211 Phospho-p38 MAPK (Thr180/Tyr182) Antibody
- 9213 p38 MAP Kinase Control Cell Extracts
- 9215 Phospho-p38 MAPK (Thr180/Tyr182) (3D7) Rabbit mAb
- 9216 Phospho-p38 MAPK (Thr180/Tyr182) (28B10) Mouse mAb
- 9219 Immobilized Phospho-p38 MAPK (Thr180/Tyr182) Mouse mAb
- 9210 PhosphoPlus® p38 MAP Kinase (Thr180/Tyr182) Antibody Kit
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
