Product Pathways - Adhesion

γ-Catenin Antibody #2309

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Applications	Reactivity	Sensitivity	MW (kDa)	Source
W IP IHC-P IF-IC	H M R Mk Hm	Endogenous	83	Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  Hm=Hamster
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

γ-Catenin Antibody detects endogenous levels ot total γ-catenin protein. The antibody does not cross-react with β-catenin.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to the carboxy terminus of human γ-catenin. Antibodies are purified using protein A and peptide affinity chromatography.

Background

Also known as plakoglobin, γ-Catenin is a member of the Armadillo family of signaling molecules, which includes β-catenin and the drosophila protein armadillo (1). This family of proteins is involved in Wnt signaling, which is important in embryonic development and in tumorigenesis (2-3). Although the two vertebrate proteins β- and γ-catenin display sequence homology, γ-catenin likely plays a role distinct from that of β-catenin (1, 4-6). γ-catenin localizes to desmosomes and adherens junctions, both sites of intercellular adhesion, and interacts with the cytoplasmic domains of classical and desmosomal cadherins. Interaction of γ- or β-catenin with α-catenin, desmoplakin and other junction proteins provides a link between intercellular junctions and the actin and intermediate filament cytoskeleton. Maintenance and/or modification of this link is vital for control of cell adhesion and migration (1). γ-catenin is modified by phosphorylation, affecting both adhesion and β-catenin dependent transcription (7), and by and O-glycosylation, affecting adhesion (8). Recent evidence suggests that γ-catenin regulates desmosomal adhesion in response to growth factor stimulation (9).

1. Zhurinsky, J. et al. (2000) J. Cell Sci. 113 ( Pt 18), 3127-3139.
2. Wodarz, A. and Nusse, R. (1998) Annu. Rev. Cell Dev. Biol. 14, 59-88.
3. Polakis, P. (1999) Curr. Opin. Genet. Dev. 9, 15-21.
4. Zhurinsky, J. et al. (2000) Mol. Cell Biol. 20, 4238-4252.
5. Charpentier, E. et al. (2000) J. Cell Biol. 149, 503-520.
6. Kolligs, F.T. et al. (2000) Genes Dev. 14, 1319-13131.
7. Miravet, S. et al. (2003) Mol. Cell Biol. 23, 7391-7402.
8. Hu, P. et al. (2006) J. Biol. Chem. 281, 12786-12791.
9. Yin, T. et al. (2005) J. Biol. Chem. 280, 40355-40363.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.