Product Pathways - Adhesion
γ-Catenin Antibody #2309
|W IP IHC-P IF-IC||H M R Hm Mk||Endogenous||83||Rabbit|
Reactivity Key: H=Human M=Mouse R=Rat Hm=Hamster Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
γ-Catenin Antibody detects endogenous levels ot total γ-catenin protein. The antibody does not cross-react with β-catenin.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of human γ-catenin. Antibodies are purified using protein A and peptide affinity chromatography.
Western blot analysis of extracts from 293, MCF-7 and C2C12 cells using γ-Catenin Antibody.
Immunohistochemical analysis of paraffin-embedded human breast carcioma using gamma-Catenin Antibody.
Immunohistochemical analysis of paraffin-embedded human colon carcioma using gamma-Catenin Antibody.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using gamma-Catenin Antibody.
Also known as plakoglobin, γ-catenin is a member of the Armadillo family of signaling molecules, which includes β-catenin and the Drosophila protein armadillo (1). This family of proteins is involved in Wnt signaling, which is important in embryonic development and in tumorigenesis (2-3). Although the two vertebrate proteins β- and γ-catenin display sequence homology, γ-catenin likely plays a role distinct from that of β-catenin (1, 4-6). γ-catenin localizes to desmosomes and adherens junctions, both sites of intercellular adhesion, and interacts with the cytoplasmic domains of classical and desmosomal cadherins. Interaction of γ- or β-catenin with α-catenin, desmoplakin and other junction proteins provides a link between intercellular junctions and the actin and intermediate filament cytoskeleton. Maintenance and/or modification of this link is vital for control of cell adhesion and migration (1). γ-catenin is modified by phosphorylation, affecting both adhesion and β-catenin dependent transcription (7), and by and O-glycosylation, affecting adhesion (8). Recent evidence suggests that γ-catenin regulates desmosomal adhesion in response to growth factor stimulation (9).
- Zhurinsky, J. et al. (2000) J. Cell Sci. 113 ( Pt 18), 3127-3139.
- Wodarz, A. and Nusse, R. (1998) Annu. Rev. Cell Dev. Biol. 14, 59-88.
- Polakis, P. (1999) Curr. Opin. Genet. Dev. 9, 15-21.
- Zhurinsky, J. et al. (2000) Mol. Cell Biol. 20, 4238-4252.
- Charpentier, E. et al. (2000) J. Cell Biol. 149, 503-520.
- Kolligs, F.T. et al. (2000) Genes Dev. 14, 1319-13131.
- Miravet, S. et al. (2003) Mol. Cell Biol. 23, 7391-7402.
- Hu, P. et al. (2006) J. Biol. Chem. 281, 12786-12791.
- Yin, T. et al. (2005) J. Biol. Chem. 280, 40355-40363.
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For Research Use Only. Not For Use In Diagnostic Procedures.