Product Pathways - Neuroscience
Synapsin Antibody #2312
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IF-F | M R (H) | Endogenous | 77 in mouse brain homogenate. | Rabbit |
Applications Key:
W=Western Blotting
IF-F=Immunofluorescence (Frozen)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Synapsin Antibody detects endogenous levels of total synapsin protein. This antibody is expected to recognize all synapsin isoforms.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues surrounding Ser9 of human synapsin I. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of mouse brain homogenates, untreated or phosphatase-treated, using Phospho-Synapsin (Ser9) Antibody #2311 (upper) or Synapsin Antibody (lower).
IF-F
Confocal microscopic image of mouse retina labeled with Synapsin Antibody (red) and CREB (86B10) Mouse mAb #9104 (green). Blue pseudeocolor stain = DRAQ5® (fluorescent DNA dye).
Background
Synapsins, a group of at least five related members (synapsins Ia, Ib, IIa, IIb and IIIa), are abundant brain proteins essential for regulating neurotransmitter release (1,2). All synapsins contain a short amino-terminal domain that is highly conserved and phosphorylated by PKA or CaM kinase I (1). Phosphorylation of synapsin amino-terminal domain at serine 9 inhibits its binding to phospholipids and dissociates synapsins from synaptic vesicles (2).
Application References
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Companion Products
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
