Product Pathways - Neuroscience
Synapsin Antibody #2312
Have you tried your application using our XP® monoclonal antibodies? Try product: 5297
PhosphoSitePlus® protein, site, and accession data: SYN1
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | M R (H) | Endogenous | 77 in mouse brain homogenate. | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 2312:
- Western Blotting
Specificity / Sensitivity
Synapsin Antibody detects endogenous levels of total synapsin protein. This antibody is expected to recognize all synapsin isoforms.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser9 of human synapsin I. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of mouse brain homogenates, untreated or phosphatase-treated, using Phospho-Synapsin (Ser9) Antibody #2311 (upper) or Synapsin Antibody (lower).
IF-F
Confocal microscopic image of mouse retina labeled with Synapsin Antibody (red) and CREB (86B10) Mouse mAb #9104 (green). Blue pseudeocolor stain = DRAQ5® (fluorescent DNA dye).
Background
Synapsins, a group of at least five related members (synapsins Ia, Ib, IIa, IIb, and IIIa), are abundant brain proteins essential for regulating neurotransmitter release (1,2). All synapsins contain a short amino-terminal domain that is highly conserved and phosphorylated by PKA or CaM kinase I (1). Phosphorylation of the synapsin amino-terminal domain at Ser9 inhibits its binding to phospholipids and dissociates synapsins from synaptic vesicles (2).
Application References
- Coufal, N.G. et al. (2011) Proc Natl Acad Sci U S A 108, 20382-7. Applications: Western Blotting
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Companion Products
- 2311 Phospho-Synapsin (Ser9) Antibody
- 2132 Parkin Antibody
- 2301 Phospho-DARPP-32 (Thr75) Antibody
- 2302 DARPP-32 Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
