Product Pathways - MAPK Signaling
c-Jun (L70B11) Mouse mAb #2315
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | H M R Mk | Endogenous | 43, 48 | Mouse IgG1 |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
c-Jun (L70B11) Mouse mAb detects endogenous levels of total c-Jun protein, regardless of phosphorylation state.
Source / Purification
Monoclonal antibody is produced by immunizing mice with GST-c-Jun partial fusion proteins.
Western Blotting
Western blot analysis of extracts from NIH/3T3 and 293 cells, untreated or UV-treated using c-Jun (L70B11) Mouse mAb.
Background
c-Jun is a component of the transcription factor AP-1, which binds and activates transcription at TRE/AP-1 elements. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73 (1,2). Extracellular signals including growth factors, transforming oncoproteins and UV irradiation stimulate phosphorylation of c-Jun at Ser63/73 and activate c-Jun-dependent transcription. Mutation of Ser63/73 renders c-Jun nonresponsive to mitogenic and stress-induced signaling pathways. The MAP kinase homologue SAPK/JNK binds to the amino-terminal region of c-Jun and phosphorylates c-Jun at Ser63/73. In addition, the activity of SAPK/JNK is stimulated by the same signals that activate c-Jun (3,4).
- Binetruy, B. et al. (1991) Nature 351, 122-127.
- Smeal, T. et al. (1991) Nature 354, 494-496.
- Derijard, B. et al. (1994) Cell 76, 1025-1037.
- Kyriakis, J.M. et al. (1994) Nature 369, 156-160.
Application References
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Companion Products
- 9260 PhosphoPlus® c-Jun (Ser63) II and c-Jun (Ser73) Antibody Kit
- 9261 Phospho-c-Jun (Ser63) II Antibody
- 9263 c-Jun Control Cell Extracts
- 9164 Phospho-c-Jun (Ser73) Antibody
- 9165 c-Jun (60A8) Rabbit mAb
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
