Product Pathways - Tyrosine Kinase / Adaptors

• pathway
• application references
• companion products
• datasheet PDF
• MSDS PDF
• protocols

NCK1 (15B9) Rabbit mAb #2319

Applications	Reactivity	Sensitivity	MW (kDa)	Isotype
W IP	H M R Mk	Endogenous	47 kDa	Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

2319:

Immunoprecipitation, Western Blotting

Specificity / Sensitivity

NCK1 (15B9) Rabbit mAb detects the endogenous levels of total NCK1 protein. Based on homology it may also recognize NCK2.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly300 of human NCK1.

Background

NCK1 (also known as NCK or NCKα) is a broadly expressed oncogenic adapter protein consisting of three SH3 domains and one SH2 domain (1-3). NCK1 becomes phosphorylated upon activation of variety of cell surface receptors and is involved in actin cytoskeletal organization induced by many stimuli (4-6). NCK2 (also known as NCKβ), a homolog of NCK1, has an overlapping expression pattern and redundant functions with NCK1 (7).

1. Lehmann, J.M. et al. (1990) Nucleic Acids Res. 18, 1048.
2. Chou, M.M. et al. (1992) Mol. Cell Biol. 12, 5834-5842.
3. Li, W. et al. (1992) Mol. Cell Biol. 12, 5824-5833.
4. Verma, R. et al. (2006) J. Clin. Invest. 116, 1346-1359.
5. Rivera, G.M. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 9536-9541.
6. Li, H. et al. (2006) Biochem. Biophys. Res. Commun. 349, 310-316.
7. Bladt, F. et al. (2003) Mol. Cell Biol. 23, 4586-4597.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

• 7071 Phototope^®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide

Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.

---

For Research Use Only. Not For Use In Diagnostic Procedures.