Product Pathways - Motif Antibodies

Phospho-(Ser) CDKs Substrate Antibody #2324

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Applications	Reactivity	Source
W IP IHC-P E-P	All	Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  E-P=ELISA (Peptide)
Reactivity Key: All=All species expected

Specificity / Sensitivity

Phospho-(Ser) CDKs Substrate Antibody detects phospho-serine in a (K/R)(S*)PX(K/R) motif. The antibody is phospho-specific but does not recognize phospho-serine in the absence of the CDK motif. The antibody does not cross-react with phospho-threonine- or phospho-tyrosine-containing peptides/proteins. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with synthetic phospho-CDK substrate peptides (KLH-coupled). Antibodies are purified by protein A and peptide affinity chromatography.

Background

Cyclin-dependent kinases (CDKs) are a family of Ser/Thr kinases that regulate cell-cycle transitions through their association and subsequent phosphorylation of targets in a strictly ordered fashion (1). The substrates for CDKs are proline-directed. The consensus amino acid sequence for CDK substrate is (K/R)(S*)PX(K/R), where X denotes any one of the 20 amino acids (2-4) and S* is the phosphorylation site. Phospho-(Ser) CDKs Substrate Antibody recognizes phosphorylated CDK substrates at their consensus motif, providing a powerful tool for CDK target discovery and characterization as well as HTS drug screening for potential kinase regulators.

1. Morgan, D.O. (1997) Annu Rev Cell Dev Biol 13, 261-91.
2. Songyang, Z. et al. (1996) Mol Cell Biol 16, 6486-93.
3. Songyang, Z. (1999) Prog Biophys Mol Biol 71, 359-72.
4. Holmes, J.K. and Solomon, M.J. (1996) J Biol Chem 271, 25240-6.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

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License/Use Restrictions: Use of CST Motif Antibodies within certain methods (e.g., U.S. Patent No.'s 7,198,896 & 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commerical licensing terms please contact CST Business Development at cbunker@cellsignal.com.