Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Rap1B (36E1) Rabbit mAb #2326

Applications Reactivity Sensitivity MW (kDa) Isotype
W H M R Mk B Pg Endogenous 21 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  B=Bovine  Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Rap1B (36E1) Rabbit mAb detects endogenous levels of total Rap1B protein. It does not cross-react with Rap1A.

Source / Purification

Monoclonal antibody is produced by immunizing animals with synthetic peptides corresponding to the carboxy terminal half of human Rap1B.

Western Blotting

Western Blotting

Western blot analysis of cell extracts from various cell lines using Rap1B (36E1) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of HeLa cell lysates using Rap1B (36E1) Rabbit mAb without pre-absorption (A), or pre-incubated with Rab1A (B) and Rap1B (C) carboxy-terminal peptides respectively. The result showed that only Rap1B peptide specifically blocks the antibody binding in Western blot.

Background

Rap1 and Rap2 belong to the Ras subfamily of small GTPases and are activated by a wide variety of stimuli through integrins, receptor tyrosine kinases (RTKs), G-protein coupled receptors (GPCR), death domain associated receptors (DD-R) and ion channels (1,2). Like other small GTPases, Rap activity is stimulated by guanine nucleotide exchange factors (GEF) and inactivated by GTPase activating proteins (GAP). A wide variety of Rap GEFs have been identified: C3G connects Rap1 with RTKs through adaptor proteins such as Crk, Epacs (or cAMP-GEFs) transmit signals from cAMP, and CD-GEFs (or CalDAG-GEFs) convey signals from either or both Ca2+ and DAG (1). Rap1 primarily regulates multiple integrin-dependent processes such as morphogenesis, cell-cell adhesion, hematopoiesis, leukocyte migration and tumor invasion (1,2). Rap1 may also regulate proliferation, differentiation and survival through downstream effectors including B-Raf, PI3K, RalGEF and phospholipases (PLCs) (1-4). Rap1 and Rap2 are not fuctionally redundant as they perform overlapping but distinct functions (5). Recent research indicates that Rap2 regulates Dsh subcellular localization and is required for Wnt signaling in early development (6).

  1. Bos, J. et al. (2001) Nat. Rev. Mol. Cell Biol. 2, 369-377.
  2. Caron, E. (2003) J. Cell Sci. 116, 435-440.
  3. Song, C. et al. (2002) Oncogene 21, 8105-8113.
  4. Rong, R. et al. (2003) J. Biol. Chem. 278, 52497-52503.
  5. Taira, K. et al. (2004) J. Biol. Chem. 279, 49488-49496.
  6. Choi, S. and Han, J. (2005) EMBO J. 24, 985-996.

Application References

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Companion Products

Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.

For Research Use Only. Not For Use In Diagnostic Procedures.

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