Product Pathways - Chromatin Regulation
Phospho-SirT1 (Ser27) Antibody #2327
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H | Endogenous | 120 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
This antibody detects endogenous levels of SirT1 protein only when phosphoryated on serine 27. The antibody does not cross-react with other sirtuin proteins.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Ser27 of human SirT1. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of 293 and HCT15 cell lysates treated or untreated with lambda phosphatase using Phospho-SirT1 (Ser27) Antibody #2327 (upper) or SirT1 Antibody #2310 (lower).
Background
The Silent Information Regulator (SIR2) family of genes is a highly conserved group of genes that encode nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylases, also known as Class III histone deacetylases. The first discovered and best characterized of these genes is Saccharomyces cerevisiae SIR2, which is involved in silencing of mating type loci, telomere maintenance, DNA damage response and cell aging (1). SirT1, the mammalian ortholog of Sir2, is a nuclear protein implicated in the regulation of many cellular processes, including apoptosis, cellular senescence, endocrine signaling, glucose homeostasis, aging and longevity. Targets of SirT1 include acetylated p53 (2,3), p300 (4), Ku70 (5), forkhead (FoxO) transcription factors (5,6), PPARγ (7) and the PPARγ coactivator-1α (PGC-1α) protein (8). Deacetylation of p53 and FoxO transcription factors represses apoptosis and increases cell survival (2,3,5,6). Deacetylation of PPARγ and PGC-1α regulates the gluconeogenic/glycolytic pathways in the liver and fat mobilization in white adipocytes in response to fasting (7,8). SirT1 deacetylase activity is inhibited by nicotinamide and activated by resveratrol. In addition, SirT1 activity may be regulated by phosphorylation, since it is phosphorylated on Ser27 and Ser47 in vivo; however, the function of these phosphorylation sites has not yet been determined (9).
- Guarente, L. (1999) Nat. Genet. 23, 281-285.
- Vaziri, H. et al. (2001) Cell 107, 149-159.
- Luo, J. et al. (2001) Cell 107, 137-148.
- Bouras, T. et al. (2005) J. Biol. Chem. 280, 10264-10276.
- Brunet, A. et al. (2004) Science 303, 2011-2015.
- Motta, M.C. et al. (2004) Cell 116, 551-563.
- Picard, F. et al. (2004) Nature 429, 771-776.
- Rodgers, J.T. et al. (2005) Nature 434, 113-118.
- Beausoleil, S.A. et al. (2004) Proc. Natl. Acad. Sci. USA 101, 12130-12135.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 2310 SirT1 Antibody
- 2314 Phospho-SirT1 (Ser47) Antibody
- 2313 SirT2 Antibody
- 2527 p53 (7F5) Rabbit mAb
- 2524 p53 (1C12) Mouse mAb
- 9282 p53 Antibody
- 2525 Acetyl-p53 (Lys382) Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 9467 FoxO3a Antibody
- 9472 FoxO4 Antibody
- 9462 FoxO1 Antibody
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
