Product Pathways - Translational Control
eEF2 Antibody #2332
|W IF-IC||H M R Mk Dm (Hm) (C)||Endogenous||95||Rabbit|
Reactivity Key: H=Human M=Mouse R=Rat Hm=Hamster Mk=Monkey C=Chicken Dm=D. melanogaster
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
eEF2 Antibody detects endogenous levels of total eEF2 independent of phosphorylation.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues at the amino-terminus of human eEF2. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from C6 cells, untreated or forskolin-treated (10 µM for 60 minutes), using Phospho-eEF2 (Thr56) Antibody (upper) or eEF2 Antibody #2332 (lower).
Eukaryotic elongation factor 2 (eEF2) catalyzes the translocation of peptidyl-tRNA from the A site to the P site on the ribosome. It has been shown that phosphorylation of eEF2 at threonine 56 by eEF2 kinase inhibits its activity (1-4). eEF2 kinase is normally dependent on Ca2+ ions and calmodulin (5,6). eEF2 kinase can also be activated by PKA in response to elevated cAMP levels (7-9), which are generally increased in stress- or starvation-related conditions. A variety of treatments known to raise intracellular Ca2+ or cAMP levels have been shown to result in increased phosphorylation of eEF2, and thus to inhibit peptide-chain elongation. The inactive phosphorylated eEF2 can be converted to its active nonphosphorylated form by a protein phosphatase, most likely a form of protein phosphatase-2A (PP-2A). Insulin, which activates protein synthesis in a wide range of cell types, induces rapid dephosphorylation of eEF2 through mTOR signaling and may involve modulation of the activity of the PP-2A or the eEF2 kinase or both (10).
- Nairn, A.C. and Palfrey, H.C. (1987) J. Biol. Chem. 262, 17299-17303.
- Ryazanov, A.G. et al. (1988) Nature 334, 170-173.
- Carlberg, U. et al. (1990) Eur. J. Biochem. 191, 639-645.
- Redpath, N.T. et al. (1993) Eur. J. Biochem. 213, 689-699.
- Nairn, A.C. et al. (1985) Proc. Natl. Acad. Sci. USA 82, 7939-7943.
- Palfrey, H.C. et al. (1987) J. Biol. Chem. 262, 9785-9792.
- Redpath, N.T. and Proud, C.G. (1993) Biochem. J. 293, 31-34.
- Diggle, T. et al. (1998) Biochem. J. 336, 525-529.
- Hovland, R. et al. (1999) FEBS Lett. 444, 97-101.
- Proud, C. (2000) Translational Control of Gene Expression. Cold Spring Harbor Laboratory Press, NY, 719-739.
- Kimball, S.R. et al. (2003) Am J Physiol Cell Physiol 284, C273-84. Applications: IC-IF
- Thomson, D.M. et al. (2010) J Appl Physiol 108, 1775-85. Applications: Western Blotting
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For Research Use Only. Not For Use In Diagnostic Procedures.