Product Pathways - MAPK Signaling
Phospho-MEK1/2 (Ser221) (166F8) Rabbit mAb #2338
| Applications | Reactivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|
| W IHC-P F | H M R Mk | 45 | Rabbit | IgG |
Applications Key:
W=Western Blotting
IHC-P=Immunohistochemistry (Paraffin)
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-MEK1/2 (Ser 221) (166F8) Rabbit mAb detects endogenous levels of MEK1/2 only when phosphorylated at serine 221.
Source / Purification
Monoclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Ser221 of human MEK1/2.
Western Blotting
Western blot analysis of extracts from HeLa and NIH/3T3 cells, untreated or TPA and UV-treated, using Phospho-MEK1/2 (Ser221) (166F8) Rabbit mAb (upper) or MEK1/2 Antibody #9122 (lower).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-MEK1/2 (Ser221)(166F8) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Phospho-MEK1/2 (Ser221) (166F8) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded prostate carcinoma, untreated (left) or lambda phosphatase-treated (right), using Phospho-MEK1/2 (Ser221) (166F8) Rabbit mAb.
Flow Cytometry
Flow cytometric analysis of Jurkat cells, untreated (blue) or PMA-treated (green), using Phospho-MEK1/2 (Ser221) (166F8) Rabbit mAb compared to a nonspecific negative control antibody (red).
Background
MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221 (in the activation loop of subdomain VIII) by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII.
- Crews, C.M. et al. (1992) Science 258, 478-480.
- Alessi, D.R. et al. (1994) EMBO J. 13, 1610-1619.
- Rosen, L.B. et al. (1994) Neuron 12, 1207-1221.
- Cowley, S. et al. (1994) Cell 77, 841-852.
Application References
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Companion Products
- 9121 Phospho-MEK1/2 (Ser217/221) Antibody
- 9122 MEK1/2 Antibody
- 9124 MEK1 Antibody
- 9125 MEK2 Antibody
- 9126 MEK1/2 (47E6) Rabbit mAb
- 9127 Phospho-MEK1 (Thr286) Antibody
- 9128 Phospho-MEK1 (Ser298) Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 1210 Phospho-MEK1/2 (Ser221) Blocking Peptide
- 9160 MEK1/2 HeLa Control Cell Extracts
- 9146 MEK1 (30C8) Rabbit mAb
- 9147 MEK2 (13E3) Rabbit mAb
- 9154 Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb
- 4694 MEK1/2 (L38C12) Mouse mAb
- 2352 MEK1 (61B12) Mouse mAb
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
