Product Pathways - MAPK Signaling
Phospho-MEK1/2 (Ser221) (166F8) Rabbit mAb #2338
|2338L||300 µl (30 western blots)||---||In Stock||---|
|2338S||100 µl (10 western blots)||---||In Stock||---|
|2338||carrier free and custom formulation / quantity||email request|
Already purchased this product? Write a Review.
|W||1:2000||Human, Mouse, Rat, Monkey||Endogenous||45||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry
Species predicted to react based on 100% sequence homology: Dog.
Specificity / Sensitivity
Phospho-MEK1/2 (Ser 221) (166F8) Rabbit mAb detects endogenous levels of MEK1/2 only when phosphorylated at serine 221.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser221 of human MEK1/2.
Western blot analysis of extracts from HeLa and NIH/3T3 cells, untreated or TPA and UV-treated, using Phospho-MEK1/2 (Ser221) (166F8) Rabbit mAb (upper) or MEK1/2 Antibody #9122 (lower).
Immunohistochemical analysis of formalin fixed, paraffin-embedded A375 cells, untreated (left) or treated with Raf1 Kinase Inhibitor 1 (right), using Phospho-MEK1/2 (Ser 221) (166F8) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-MEK1/2 (Ser221)(166F8) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Phospho-MEK1/2 (Ser221) (166F8) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded prostate carcinoma, untreated (left) or lambda phosphatase-treated (right), using Phospho-MEK1/2 (Ser221) (166F8) Rabbit mAb.
Flow cytometric analysis of Jurkat cells, untreated (blue) or PMA-treated (green), using Phospho-MEK1/2 (Ser221) (166F8) Rabbit mAb compared to a nonspecific negative control antibody (red).
MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC-12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII.
- Fei, Z. et al. (2011) J Biol Chem 286, 27761-8. Applications: Western Blotting.
- Falco, A. et al. (2012) Oncogene , . Applications: Western Blotting.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 8112 SignalStain® Antibody Diluent
- 1210 Phospho-MEK1/2 (Ser221) Blocking Peptide
- 9903 U0126
- 9160 MEK1/2 HeLa Control Cell Extracts
- 9120 PhosphoPlus® MEK1/2 (Ser217/221) Antibody Kit
- 9154 Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb
- 9126 MEK1/2 (47E6) Rabbit mAb
- 4694 MEK1/2 (L38C12) Mouse mAb
- 2352 MEK1 (61B12) Mouse mAb
- 9146 MEK1 (30C8) Rabbit mAb
- 9147 MEK2 (13E3) Rabbit mAb
- 9121 Phospho-MEK1/2 (Ser217/221) Antibody
- 9122 MEK1/2 Antibody
- 9124 MEK1 Antibody
- 9125 MEK2 Antibody
- 9127 Phospho-MEK1 (Thr286) Antibody
- 9128 Phospho-MEK1 (Ser298) Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 8211 PhosphoPlus® MEK1/2 (Ser217/Ser221) Antibody Duet
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
This antibody is developed, validated, and produced by CST using in part technology under license (granting certain rights including those under U.S. Patents No. 5,675,063 and 7,429,487) from Epitomics, Inc.