Product Pathways - DNA Damage

Phospho-Chk1 (Ser345) Antibody #2341

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Applications	Reactivity	MW (kDa)	Source
W F	H M R Mk Pg	56	Rabbit

Applications Key:  W=Western Blotting  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  Pg=Pig
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Phospho-Chk1 (Ser345) Antibody detects endogenous levels of Chk1 only when phosphorylated at serine 345. This antibody does not cross-react with Chk1 when phosphorylated at other sites.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Ser345 of human Chk1. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Chk1 kinase acts downstream of ATM/ATR kinase to play an important role in DNA damage checkpoint control, embryonic development and tumor suppression (1). Activation of Chk1 involves phosphorylation of Ser317 and Ser345 and occurs in response to blocked DNA replication and certain forms of genotoxic stress (2). Chk1 is also phosphorylated at Ser280 and Ser296 following DNA damage. Activated Chk1 can inactivate cdc25C via phosphorylation at Ser216, blocking the activation of cdc2 and transition into mitosis (3). Chk1 can also phosphorylate p53 at Ser20 in vitro (4).

1. Martinho, R.G. et al. (1998) EMBO J. 17, 7239-7249.
2. Zhao, H. et al. (2001) Mol. Cell. Biol. 21, 4129-4139.
3. Zeng, Y. et al. (1998) Nature 395, 507-510.
4. Shieh, S. et al. (2000) Genes Dev. 14, 289-300.

Application References

• Cliby, W. A. et al. (2002) S phase and G2 arrests induced by topoisomerase I poisons are dependent on ATR kinase function. J. Biol. Chem. 277 (2), 1599-1606. This article references the use of Phospho-Chk1 (Ser345) Antibody in the following applications: Western Blotting
• Castedo, M. et al. (2004) The cell cycle checkpoint kinase Chk2 is a negative regulator of mitotic catastrophe. Oncogene 23, 4353-4361. This article references the use of Phospho-Chk1 (Ser345) Antibody in the following applications: Western Blotting
• d'Adda di Fagagna, F. et al. (2003) A DNA damage checkpoint response in telomere-initiated senescence. Nature 426, 194-198. This article references the use of Phospho-Chk1 (Ser345) Antibody in the following applications: Western Blotting
• Fang, Y. et al. (2004) ATR functions as a gene dosage-dependent tumor suppressor on a mismatch repair-deficient background. EMBO J. 23, 3164-3174. This article references the use of Phospho-Chk1 (Ser345) Antibody in the following applications: Western Blotting
• Shibata, Y. and Nakamura, T. (2002) Defective flap endonuclease 1 activity in mammalian cells is associated with impaired DNA repair and prolonged S phase delay. J. Biol. Chem. 277, 746-754. This article references the use of Phospho-Chk1 (Ser345) Antibody in the following applications: Western Blot
• Deming , P. B. et al. (2001) The human decatenation checkpoint. Proc. Natl. Acad. Sci. USA 98, 12044-12049. This article references the use of Phospho-Chk1 (Ser345) Antibody in the following applications: Western Blot

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