Product Pathways - Cytoskeletal Signaling
Dynamin I/II Antibody #2342
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H M R | Endogenous | 100 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
Dynamin I/II Antibody detects endogenous levels of total dynamin protein. The antibody does not recognize the related dynamin-like protein DLP.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr231 of human dynamin. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from NIH/3T3, PC12 and HEK293 cells using Dynamin I/II Antibody.
IP
Dynamin was immunoprecipitated from PC12 and HEK293 cells followed by western blot analysis using Dynamin I/II Antibody.
Background
Dynamin is a family of large GTPases that has been implicated in the formation of vesicles of both the endocytotic and secretory processes (1). Dynamin plays an important role in the internalization of cell surface receptors, a process that attenuates the response to extracellular signals. It has been illustrated that dynamin interacts with signaling proteins such as Src, PLCγ, PKC and G-proteins. PKC and Src phosphorylate dynamin, and its phosphorylation may regulate the endocytosis of cell surface receptors (2,3).
- McNiven, M.A. et al. (2000) Trends Biochem. Sci. 25, 115-120.
- Powell, K.A. et al. (2000) J. Biol. Chem. 275, 11610-11617.
- Ahn, S. et al. (1999) J. Biol. Chem. 274, 1185-1188.
Application References
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Companion Products
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
