Product Pathways - DNA Damage
Phospho-Chk1 (Ser317) Antibody #2344
| Applications | Reactivity | MW (kDa) | Source |
|---|---|---|---|
| W | H R Mk Mi | 56 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
R=Rat
Mk=Monkey
Mi=Mink
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-Chk1 (Ser317) Antibody detects endogenous levels of Chk1 only when phosphorylated at Ser317. The antibody does not recognize Chk1 phosphorylated at other sites.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Ser317 of human Chk1. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from HeLa and COS cells treated with HU or UV as indicated, using Phospho-Chk1 (Ser317) Antibody.
Western Blotting
Western blot analysis of extracts from MvILu cells treated with UV or HU as indicated, using Phospho-Chk1 (Ser317) Antibody.
Background
Chk1 kinase acts downstream of ATM/ATR kinase to play an important role in DNA damage checkpoint control, embryonic development and tumor suppression (1). Activation of Chk1 involves phosphorylation of Ser317 and Ser345 and occurs in response to blocked DNA replication and certain forms of genotoxic stress (2). Chk1 is also phosphorylated at Ser280 and Ser296 following DNA damage. Activated Chk1 can inactivate cdc25C via phosphorylation at Ser216, blocking the activation of cdc2 and transition into mitosis (3). Chk1 can also phosphorylate p53 at Ser20 in vitro (4).
- Martinho, R.G. et al. (1998) EMBO J. 17, 7239-7249.
- Zhao, H. et al. (2001) Mol. Cell. Biol. 21, 4129-4139.
- Zeng, Y. et al. (1998) Nature 395, 507-510.
- Shieh, S. et al. (2000) Genes Dev. 14, 289-300.
Application References
- Heffernan , T. P. et al. (2002) An ATR- and Chk1-dependent S checkpoint inhibits replicon initiation following UVC-induced DNA damage. Mol. Cell Biol. 22, 8552-8561. This article references the use of Phospho-Chk1 (Ser317) Antibody in the following applications: Western Blotting
- Ward, I. M. et al. (2004) UV-induced ataxia-telangiectasia-mutated and Rad3-related (ATR) activation requires replication stress. J. Biol. Chem. 279, 9677-9680. This article references the use of Phospho-Chk1 (Ser317) Antibody in the following applications: IC-IF Western Blotting
- Chini, C.C. and Chen, J. (2003) Human claspin is required for replication checkpoint control. J. Biol. Chem. 278, 30057-30062. This article references the use of Phospho-Chk1 (Ser317) Antibody in the following applications: IC-ABC Western Blotting
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