Product Pathways - DNA Damage
Chk1 Antibody #2345
| Applications | Reactivity | MW (kDa) | Source |
|---|---|---|---|
| W | H M R Mk Mi | 56 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Mi=Mink
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Chk1 Antibody detects endogenous levels of total Chk1 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues surrounding Ser296 of human Chk1. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from 293, MvILu, C6, HeLa and COS cells, using Chk1 Antibody.
Background
Chk1 kinase acts downstream of ATM/ATR kinase to play an important role in DNA damage checkpoint control, embryonic development and tumor suppression (1). Activation of Chk1 involves phosphorylation of Ser317 and Ser345 and occurs in response to blocked DNA replication and certain forms of genotoxic stress (2). Chk1 is also phosphorylated at Ser280 and Ser296 following DNA damage. Activated Chk1 can inactivate cdc25C via phosphorylation at Ser216, blocking the activation of cdc2 and transition into mitosis (3). Chk1 can also phosphorylate p53 at Ser20 in vitro (4).
- Martinho, R.G. et al. (1998) EMBO J. 17, 7239-7249.
- Zhao, H. et al. (2001) Mol. Cell. Biol. 21, 4129-4139.
- Zeng, Y. et al. (1998) Nature 395, 507-510.
- Shieh, S. et al. (2000) Genes Dev. 14, 289-300.
Application References
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