Cell Signaling Technology

Product Pathways - DNA Damage

Phospho-Chk1 (Ser345) (133D3) Rabbit mAb #2348

Applications Reactivity MW (kDa) Source Isotype
W H M R 56 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Phospho-Chk1 (Ser345) (133D3) Rabbit mAb detects endogenous levels of Chk1 only when phosphorylated at serine 345.

Source / Purification

Monoclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Ser345 of human Chk1.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, COS, NIH/3T3 and C6 cells, untreated or UV-treated, using Phospho-Chk1 (Ser345) (133D30) Rabbit mAb.

Background

Chk1 kinase acts downstream of ATM/ATR kinase to play an important role in DNA damage checkpoint control, embryonic development and tumor suppression (1). Activation of Chk1 involves phosphorylation of Ser317 and Ser345 and occurs in response to blocked DNA replication and certain forms of genotoxic stress (2). Chk1 is also phosphorylated at Ser280 and Ser296 following DNA damage. Activated Chk1 can inactivate cdc25C via phosphorylation at Ser216, blocking the activation of cdc2 and transition into mitosis (3). Chk1 can also phosphorylate p53 at Ser20 in vitro (4).

  1. Martinho, R.G. et al. (1998) EMBO J. 17, 7239-7249.
  2. Zhao, H. et al. (2001) Mol. Cell. Biol. 21, 4129-4139.
  3. Zeng, Y. et al. (1998) Nature 395, 507-510.
  4. Shieh, S. et al. (2000) Genes Dev. 14, 289-300.

Application References

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Companion Products

Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.

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