Product Pathways - DNA Damage
Phospho-Chk1 (Ser345) (133D3) Rabbit mAb #2348
| Applications | Reactivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|
| W | H M R | 56 | Rabbit | IgG |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-Chk1 (Ser345) (133D3) Rabbit mAb detects endogenous levels of Chk1 only when phosphorylated at serine 345.
Source / Purification
Monoclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Ser345 of human Chk1.
Western Blotting
Western blot analysis of extracts from HeLa, COS, NIH/3T3 and C6 cells, untreated or UV-treated, using Phospho-Chk1 (Ser345) (133D30) Rabbit mAb.
Background
Chk1 kinase acts downstream of ATM/ATR kinase to play an important role in DNA damage checkpoint control, embryonic development and tumor suppression (1). Activation of Chk1 involves phosphorylation of Ser317 and Ser345 and occurs in response to blocked DNA replication and certain forms of genotoxic stress (2). Chk1 is also phosphorylated at Ser280 and Ser296 following DNA damage. Activated Chk1 can inactivate cdc25C via phosphorylation at Ser216, blocking the activation of cdc2 and transition into mitosis (3). Chk1 can also phosphorylate p53 at Ser20 in vitro (4).
- Martinho, R.G. et al. (1998) EMBO J. 17, 7239-7249.
- Zhao, H. et al. (2001) Mol. Cell. Biol. 21, 4129-4139.
- Zeng, Y. et al. (1998) Nature 395, 507-510.
- Shieh, S. et al. (2000) Genes Dev. 14, 289-300.
Application References
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Companion Products
- 2341 Phospho-Chk1 (Ser345) Antibody
- 2344 Phospho-Chk1 (Ser317) Antibody
- 2345 Chk1 Antibody
- 2347 Phospho-Chk1(Ser280) Antibody
- 6240 SignalSilence® Chk1 siRNA Kit (Human Specific)
- 6241 SignalSilence® Chk1 siRNA (Human Specific)
- 9931 Phospho-Chk1/2 Antibody Sampler Kit
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
