Cell Signaling Technology

Product Pathways - DNA Damage

Phospho-Chk1 (Ser296) Antibody #2349

Applications Reactivity MW (kDa) Source
W H M R Mk Mi 56 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  Mi=Mink
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Phospho-Chk1 (Ser296) Antibody detects endogenous levels of Chk1 only when phosphorylated at serine 296. The antibody does not recognize Chk1 phosphorylated at other sites.

Source / Purification

Antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Ser296 of human Chk1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 and MvlLu cells treated with UV or hydroxyurea (HU) as indicated, using Phospho-Chk1 (Ser296) Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from C6 and COS cells treated with UV or hydroxyurea (HU) as indicated, using Phospho-Chk1 (Ser296) Antibody.

Background

Chk1 kinase acts downstream of ATM/ATR kinase to play an important role in DNA damage checkpoint control, embryonic development and tumor suppression (1). Activation of Chk1 involves phosphorylation of Ser317 and Ser345 and occurs in response to blocked DNA replication and certain forms of genotoxic stress (2). Chk1 is also phosphorylated at Ser280 and Ser296 following DNA damage. Activated Chk1 can inactivate cdc25C via phosphorylation at Ser216, blocking the activation of cdc2 and transition into mitosis (3). Chk1 can also phosphorylate p53 at Ser20 in vitro (4).

  1. Martinho, R.G. et al. (1998) EMBO J. 17, 7239-7249.
  2. Zhao, H. et al. (2001) Mol. Cell. Biol. 21, 4129-4139.
  3. Zeng, Y. et al. (1998) Nature 395, 507-510.
  4. Shieh, S. et al. (2000) Genes Dev. 14, 289-300.

Application References

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