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HA-Tag (6E2) Mouse mAb (Alexa Fluor® 488 Conjugate) #2350
| Applications | Reactivity | Sensitivity | Source | Isotype |
|---|---|---|---|---|
| IF-IC F | All | Transfected Only | Mouse | IgG1 |
Applications Key:
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key: All=All species expected
Specificity / Sensitivity
HA-Tag (6E2) Mouse mAb (Alexa Fluor® 488 Conjugate) detects transfected HA-tagged proteins. The antibody recognizes the HA-tag fused to either the amino or carboxy terminus of targeted proteins in transfected mammalian cells.
Source / Purification
Monoclonal antibody is produced by immunizing mice with a synthetic peptide (KLH-coupled) containing the influenza hemagglutinin epitope (YPYDVPDYA).The antibody was conjugated to Alexa Fluor® 488 under optimal conditions with an F/P ratio of 2-6.
Flow Cytometry
Flow cytometric analysis of COS cells, untransfected (blue) or transfected with HA-tagged Akt3 (green), using HA-Tag (6E2) Mouse mAb (Alexa Fluor® 488 Conjugate).
IF-IC
Immunofluorescent staining of COS cells, without transfection (A) or transfected with HA-tagged Akt3 (B), using HA-Tag (6E2) Mouse mAb (Alexa Fluor® 488 Conjugate).
Description
Cell Signaling Technology Antibody was conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometry and immunofuorescence in cells transfected with HA-tagged protein.
Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.
The HA tag is derived from an epitope of the influenza hemagglutinin protein, which has been extensively used as a general epitope tag in expression vectors (1).
- Field, J. et al. (1988) Mol. Cell. Biol. 8, 2159-2165.
Application References
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Companion Products
Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
