Product Pathways - MAPK Signaling
MEK1 (61B12) Mouse mAb #2352
|W IP||H M R Mk||Endogenous||45 kDa||Mouse IgG2a|
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
MEK1 (61B2) Mouse mAb detects endogenous levels of total MEK1 protein. This antibody does not cross-react with MEK2 and other MAP kinase kinases.
Source / Purification
Monoclonal antibody is produced by immunizing animals with full length MEK1 proteins.
Western blot analysis of extracts from HeLa, NIH/3T3 and A431 cells, using MEK1 (61B2) Mouse mAb.
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence® MEK1 siRNA I #6426 or SignalSilence® MEK1 siRNA II #6530 (+), using MEK1 (61B12) Mouse mAb #2352 and α-Tubulin (11H10) Rabbit mAb #2125. The MEK1 (61B12) Mouse mAb confirms silencing of MEK1 expression and α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of MEK1 siRNA.
MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC-12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII.
- Crews, C.M. et al. (1992) Science 258, 478-480.
- Alessi, D.R. et al. (1994) EMBO J. 13, 1610-1619.
- Rosen, L.B. et al. (1994) Neuron 12, 1207-1221.
- Cowley, S. et al. (1994) Cell 77, 841-852.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 9900 PD98059
- 9903 U0126
- 9160 MEK1/2 HeLa Control Cell Extracts
- 9120 PhosphoPlus® MEK1/2 (Ser217/221) Antibody Kit
- 9154 Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb
- 2338 Phospho-MEK1/2 (Ser221) (166F8) Rabbit mAb
- 9126 MEK1/2 (47E6) Rabbit mAb
- 4694 MEK1/2 (L38C12) Mouse mAb
- 9146 MEK1 (30C8) Rabbit mAb
- 9147 MEK2 (13E3) Rabbit mAb
- 9121 Phospho-MEK1/2 (Ser217/221) Antibody
- 9122 MEK1/2 Antibody
- 9124 MEK1 Antibody
- 9125 MEK2 Antibody
- 9127 Phospho-MEK1 (Thr286) Antibody
- 9128 Phospho-MEK1 (Ser298) Antibody
- 7072 Phototope®-HRP Western Blot Detection System, Anti-mouse IgG, HRP-linked Antibody
- 7076 Anti-mouse IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.