Cell Signaling Technology

Product Pathways - MAPK Signaling

MEK1 (61B12) Mouse mAb #2352

Applications Reactivity MW (kDa) Source Isotype
W IP H M R Mk 45 kDa Mouse IgG1

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

MEK1 (61B2) Mouse mAb detects endogenous levels of total MEK1 protein. This antibody does not cross-react with MEK2 and other MAP kinase kinases.

Source / Purification

Monoclonal antibodies are produced by immunizing mice with full length MEK1 proteins.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, NIH/3T3 and A431 cells, using MEK1 (61B2) Mouse mAb.

Background

MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221 (in the activation loop of subdomain VIII) by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII.

  1. Crews, C.M. et al. (1992) Science 258, 478-480.
  2. Alessi, D.R. et al. (1994) EMBO J. 13, 1610-1619.
  3. Rosen, L.B. et al. (1994) Neuron 12, 1207-1221.
  4. Cowley, S. et al. (1994) Cell 77, 841-852.

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