Cell Signaling Technology

Product Pathways - DNA Damage

Chk1 (2G1D5) Mouse mAb #2360

Applications Reactivity MW (kDa) Source Isotype
W IF-IC F H M R 56 Mouse IgG1

Applications Key:  W=Western Blotting  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Chk1 (2G1D5) Mouse mAb recognizes endogenous levels of total Chk1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing mice with purified recombinant Chk1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines, using Chk1 (2G1D5) Mouse mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells, using Chk1 (2G1D5) Mouse mAb (blue) compared to a nonspecific negative control antibody (red).

IF-IC

IF-IC

Immunofluorescent analysis of HeLa cells, untreated (left) or UV-treated (right), using Chk1 (2G1D5) Mouse mAb.


Background

Chk1 kinase acts downstream of ATM/ATR kinase to play an important role in DNA damage checkpoint control, embryonic development and tumor suppression (1). Activation of Chk1 involves phosphorylation of Ser317 and Ser345 and occurs in response to blocked DNA replication and certain forms of genotoxic stress (2). Chk1 is also phosphorylated at Ser280 and Ser296 following DNA damage. Activated Chk1 can inactivate cdc25C via phosphorylation at Ser216, blocking the activation of cdc2 and transition into mitosis (3). Chk1 can also phosphorylate p53 at Ser20 in vitro (4).

  1. Martinho, R.G. et al. (1998) EMBO J. 17, 7239-7249.
  2. Zhao, H. et al. (2001) Mol. Cell. Biol. 21, 4129-4139.
  3. Zeng, Y. et al. (1998) Nature 395, 507-510.
  4. Shieh, S. et al. (2000) Genes Dev. 14, 289-300.

Application References

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