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HA-Tag (6E2) Mouse mAb #2367
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| W IHC-P IF-IC F | All | Transfected Only | Mouse IgG1 |
Applications Key:
W=Western Blotting
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key: All=All species expected
Protocols
Specificity / Sensitivity
HA-Tag (6E2) Mouse mAb detects recombinant proteins containing the HA epitope tag. The antibody recognizes the HA-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide containing the influenza hemagglutinin epitope (YPYDVPDYA) .
Western Blotting
Western blot analysis of extracts from COS cells, untransfected (lane 1) or expressing HA-tagged Akt3 (lane 2) or HA-tagged Estrogen Receptor (ER) (lane 3), using HA-Tag (6E2) Mouse mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded COS cells, untransfected (left), HA-Akt3 transfected (middle) or HA-Estrogen Receptor transfected (right), using an Akt antibody (top), an Estrogen Receptor antibody (middle) or HA-Tag (6E2) Mouse mAb (bottom).
Flow Cytometry
Flow cytometric analysis of COS cells, untransfected (blue) or transfected with HA-Akt3 (green), using HA-Tag (6E2) Mouse mAb compared to a nonspecific negative control antibody (red).
IF-IC
Immunocytochemical staining of COS cells without transfection (left), expressing HA-tagged Akt3 (middle) or expressing HA-tagged Estrogen Receptor (right), using HA-Tag (6E2) Mouse mAb.
IF-IC
Confocal immunofluorescent analysis of COS cells, mock-transfected (left) or transfected with HA-tagged protein (right), using HA-Tag (6E2) Mouse mAb (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Background
The HA tag is derived from an epitope of the influenza hemagglutinin protein, which has been used extensively as a general epitope tag in expression vectors (1).
- Field, J. et al. (1988) Mol. Cell. Biol. 8, 2159-2165.
Application References
- Klein, R.M. et al. (2008) Mol Biol Cell 19, 498-508. Applications: IF-IC (In Cells)
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
