Product Pathways - Related Products
HA-Tag (6E2) Mouse mAb #2367
| Applications | Reactivity | Sensitivity | Source | Isotype |
|---|---|---|---|---|
| W IP IHC-P IF-IC F | All | Transfected Only | Mouse | IgG1 |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key: All=All species expected
Specificity / Sensitivity
HA-Tag (6E2) Mouse mAb detects transfected HA-tagged proteins. The antibody recognizes the HA-tag fused to either the amino- or carboxy-terminus of targeted proteins in transfected mammalian cells.
Source / Purification
Monoclonal antibody is produced by immunizing mice with a synthetic peptide containing the influenza hemagglutinin epitope (YPYDVPDYA) (KLH-coupled).
Western Blotting
Western blot analysis of extracts from COS cells, untransfected (lane 1) or expressing HA-tagged Akt3 (lane 2) or HA-tagged Estrogen Receptor (ER) (lane 3), using HA-Tag (6E2) Mouse mAb.
IP
Immunoprecipitation of extracts from untransfected COS cells (lanes 2 and 5) or COS cells expressing HA-tagged Akt3 (lane 3) or HA-tagged Estrogen Receptor (ER) (lane 6), using HA-Tag (6E2) Mouse mAb followed by Western blot analysis using Akt Antibody #9272 (lanes 1-3) or Estrogen Receptor alpha (62A3) Mouse mAb #2512 (lanes 4-6).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded COS cells, untransfected (left), HA-Akt3 transfected (middle) or HA-Estrogen Receptor transfected (right), using an Akt antibody (top), an Estrogen Receptor antibody (middle) or HA-Tag (6E2) Mouse mAb (bottom).
Flow Cytometry
Flow cytometric analysis of COS cells, untransfected (blue) or transfected with HA-Akt3 (green), using HA-Tag (6E2) Mouse mAb compared to a nonspecific negative control antibody (red).
IF-IC
Immunocytochemical staining of COS cells without transfection (left), expressing HA-tagged Akt3 (middle) or expressing HA-tagged Estrogen Receptor (right), using HA-Tag (6E2) Mouse mAb.
Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.
The HA tag is derived from an epitope of the influenza hemagglutinin protein, which has been extensively used as a general epitope tag in expression vectors (1).
- Field, J. et al. (1988) Mol. Cell. Biol. 8, 2159-2165.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 2350 HA-Tag (6E2) Mouse mAb (Alexa Fluor® 488 Conjugate)
- 2272 Myc-Tag Antibody
- 2276 Myc-Tag (9B11) Mouse mAb
- 2365 His-Tag Antibody
- 2366 His-Tag (27E8) Mouse mAb
- 2372 β-Gal (14B7) Mouse mAb
- 2396 MBP (8G1) Mouse mAb
- 7072 Phototope®-HRP Western Blot Detection System, Anti-mouse IgG, HRP-linked Antibody
- 7076 Anti-mouse IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
