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HA-Tag (6E2) Mouse mAb #2367
| Applications | Reactivity | Sensitivity | Source | Isotype |
|---|---|---|---|---|
| W IP IHC-P IF-IC F | All | Transfected Only | Mouse | IgG1 |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key: All=All species expected
Specificity / Sensitivity
HA-Tag (6E2) Mouse mAb detects transfected HA-tagged proteins. The antibody recognizes the HA-tag fused to either the amino- or carboxy-terminus of targeted proteins in transfected mammalian cells.
Source / Purification
Monoclonal antibody is produced by immunizing mice with a synthetic peptide containing the influenza hemagglutinin epitope (YPYDVPDYA) (KLH-coupled).
Western Blotting
Western blot analysis of extracts from COS cells, untransfected (lane 1) or expressing HA-tagged Akt3 (lane 2) or HA-tagged Estrogen Receptor (ER) (lane 3), using HA-Tag (6E2) Mouse mAb.
IP
Immunoprecipitation of extracts from untransfected COS cells (lanes 2 and 5) or COS cells expressing HA-tagged Akt3 (lane 3) or HA-tagged Estrogen Receptor (ER) (lane 6), using HA-Tag (6E2) Mouse mAb followed by Western blot analysis using Akt Antibody #9272 (lanes 1-3) or Estrogen Receptor alpha (62A3) Mouse mAb #2512 (lanes 4-6).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded COS cells, untransfected (left), HA-Akt3 transfected (middle) or HA-Estrogen Receptor transfected (right), using an Akt antibody (top), an Estrogen Receptor antibody (middle) or HA-Tag (6E2) Mouse mAb (bottom).
Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.
The HA tag is derived from an epitope of the influenza hemagglutinin protein, which has been extensively used as a general epitope tag in expression vectors (1).
- Field, J. et al. (1988) Mol. Cell. Biol. 8, 2159-2165.
Application References
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.