Product Pathways - Companion Products
HA-Tag (6E2) Mouse mAb #2367
|W IHC-P IF-IC F||All||Transfected Only||Mouse IgG1|
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.
Specificity / Sensitivity
HA-Tag (6E2) Mouse mAb detects recombinant proteins containing the HA epitope tag. The antibody recognizes the HA-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide containing the influenza hemagglutinin epitope (YPYDVPDYA) .
Western blot analysis of extracts from COS cells, untransfected (lane 1) or expressing HA-tagged Akt3 (lane 2) or HA-tagged Estrogen Receptor (ER) (lane 3), using HA-Tag (6E2) Mouse mAb.
Immunohistochemical analysis of paraffin-embedded COS cells, untransfected (left), HA-Akt3 transfected (middle) or HA-Estrogen Receptor transfected (right), using an Akt antibody (top), an Estrogen Receptor antibody (middle) or HA-Tag (6E2) Mouse mAb (bottom).
Flow cytometric analysis of COS cells, untransfected (blue) or transfected with HA-Akt3 (green), using HA-Tag (6E2) Mouse mAb compared to a nonspecific negative control antibody (red).
Immunocytochemical staining of COS cells without transfection (left), expressing HA-tagged Akt3 (middle) or expressing HA-tagged Estrogen Receptor (right), using HA-Tag (6E2) Mouse mAb.
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.
The HA tag is derived from an epitope of the influenza hemagglutinin protein, which has been used extensively as a general epitope tag in expression vectors (1).
- Field, J. et al. (1988) Mol. Cell. Biol. 8, 2159-2165.
- Klein, R.M. et al. (2008) Mol Biol Cell 19, 498-508. Applications: IF-IC (In Cells)
- Pfeffer, L.A. et al. (2009) Mol Biol Cell 20, 3810-7. Applications: IF-IC (In Cells)
- Volná, P. et al. (2007) Nucleic Acids Res 35, 2748-58. Applications: Western Blotting
- Jacquet, S. et al. (2008) J Biol Chem 283, 11964-71. Applications: Western Blotting
- Lim, S. et al. (2009) Mol Cancer Res 7, 55-66. Applications: IF-IC (In Cells) Western Blotting
- Corcoran, K. et al. (2011) J Biol Chem 286, 37168-80. Applications: IF-IC (In Cells)
- Joost, S. et al. (2012) Cancer Res 72, 88-99. Applications: Western Blotting
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For Research Use Only. Not For Use In Diagnostic Procedures.