Product Pathways - Companion Products
HA-Tag (6E2) Mouse mAb #2367
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| W IHC-P IF-IC F | All | Transfected Only | Mouse IgG1 |
Applications Key:
W=Western Blotting
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.
Protocols
Specificity / Sensitivity
HA-Tag (6E2) Mouse mAb detects recombinant proteins containing the HA epitope tag. The antibody recognizes the HA-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide containing the influenza hemagglutinin epitope (YPYDVPDYA) .
Western Blotting
Western blot analysis of extracts from COS cells, untransfected (lane 1) or expressing HA-tagged Akt3 (lane 2) or HA-tagged Estrogen Receptor (ER) (lane 3), using HA-Tag (6E2) Mouse mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded COS cells, untransfected (left), HA-Akt3 transfected (middle) or HA-Estrogen Receptor transfected (right), using an Akt antibody (top), an Estrogen Receptor antibody (middle) or HA-Tag (6E2) Mouse mAb (bottom).
Flow Cytometry
Flow cytometric analysis of COS cells, untransfected (blue) or transfected with HA-Akt3 (green), using HA-Tag (6E2) Mouse mAb compared to a nonspecific negative control antibody (red).
IF-IC
Immunocytochemical staining of COS cells without transfection (left), expressing HA-tagged Akt3 (middle) or expressing HA-tagged Estrogen Receptor (right), using HA-Tag (6E2) Mouse mAb.
IF-IC
Confocal immunofluorescent analysis of COS cells, mock-transfected (left) or transfected with HA-tagged protein (right), using HA-Tag (6E2) Mouse mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.
The HA tag is derived from an epitope of the influenza hemagglutinin protein, which has been used extensively as a general epitope tag in expression vectors (1).
- Field, J. et al. (1988) Mol. Cell. Biol. 8, 2159-2165.
Application References
- Klein, R.M. et al. (2008) Mol Biol Cell 19, 498-508. Applications: IF-IC (In Cells)
- Pfeffer, L.A. et al. (2009) Mol Biol Cell 20, 3810-7. Applications: IF-IC (In Cells)
- Volná, P. et al. (2007) Nucleic Acids Res 35, 2748-58. Applications: Western Blotting
- Jacquet, S. et al. (2008) J Biol Chem 283, 11964-71. Applications: Western Blotting
- Lim, S. et al. (2009) Mol Cancer Res 7, 55-66. Applications: IF-IC (In Cells) Western Blotting
- Corcoran, K. et al. (2011) J Biol Chem 286, 37168-80. Applications: IF-IC (In Cells)
- Joost, S. et al. (2012) Cancer Res 72, 88-99. Applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 2350 HA-Tag (6E2) Mouse mAb (Alexa Fluor® 488 Conjugate)
- 2272 Myc-Tag Antibody
- 2276 Myc-Tag (9B11) Mouse mAb
- 2956 GFP (D5.1) XP® Rabbit mAb
- 2365 His-Tag Antibody
- 2366 His-Tag (27E8) Mouse mAb
- 2372 β-Gal (14B7) Mouse mAb
- 2396 MBP (8G1) Mouse mAb
- 7072 Phototope®-HRP Western Blot Detection System, Anti-mouse IgG, HRP-linked Antibody
- 7076 Anti-mouse IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 3724 HA-Tag (C29F4) Rabbit mAb
For Research Use Only. Not For Use In Diagnostic Procedures.
