Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Villin-1 (R814) Antibody #2369

Applications Reactivity Sensitivity MW (kDa) Source
W IF-IC H M (R) Endogenous 95 Rabbit

Applications Key:  W=Western Blotting  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Villin-1 (R814) Antibody recognizes endogenous levels of total villin-1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of human villin-1. Antibodies are purified using protein A and peptide affinitty chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HT29 cells and mouse kidney using Villin-1 (R814) Antibody.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HT29 cells using Villin-1 (R814) Antibody (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

Background

Villin is a member of the gelsolin family of calcium-regulated actin-binding proteins. Unlike the ubiquitously expressed gelsolin, villin expression is restricted to simple epithelia of the gastrointestinal and urogenital tracts. It is localized to the apical cytoplasm and brush borders of these cells. Villin functions in the regulation of actin dynamics in the apical epithelium, capping, nucleating and/or severing actin filaments in a calcium-dependent manner and regulating cell shape in response to external stimuli (1,2). Phosphorylation of villin at Tyr60, 81 and 256 may be involved in the regulation of cell migration (3). Expression of villin is increased in colorectal cancers (4), and villin1 function appears to be involved in progressive cholestasis and hepatic failure (5).

  1. Athman, R. et al. (2002) Am. J. Physiol. Gastrointest. Liver Physiol. 283, G496-502.
  2. Kumar, N. et al. (2004) J. Biol. Chem. 279, 45036-45046.
  3. Tomar, A. et al. (2004) Mol. Biol. Cell 15, 4807-4817.
  4. Suh, N. et al. (2005) Mod. Pathol. 18, 1217-1222.
  5. Phillips, M.J. et al. (2003) Lancet 362, 1112-1119.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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