Product Pathways - Cytoskeletal Signaling
Villin-1 (R814) Antibody #2369
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IF-IC | H M (R) | Endogenous | 95 | Rabbit |
Applications Key:
W=Western Blotting
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Villin-1 (R814) Antibody recognizes endogenous levels of total villin-1 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to the carboxy terminus of human villin-1. Antibodies are purified using protein A and peptide affinitty chromatography.
Western Blotting
Western blot analysis of extracts from HT29 cells and mouse kidney using Villin-1 (R814) Antibody.
IF-IC
Confocal immunofluorescent analysis of HT29 cells using Villin-1 (R814) Antibody (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Background
Villin is a member of the gelsolin family of calcium-regulated actin-binding proteins. Unlike the ubiquitously expressed gelsolin, villin expression is restricted to simple epithelia of the gastrointestinal and urogenital tracts. It is localized to the apical cytoplasm and brush borders of these cells. Villin functions in the regulation of actin dynamics in the apical epithelium, capping, nucleating and/or severing actin filaments in a calcium-dependent manner and regulating cell shape in response to external stimuli (1,2). Phosphorylation of villin at Tyr60, 81 and 256 may be involved in the regulation of cell migration (3). Expression of villin is increased in colorectal cancers (4), and villin1 function appears to be involved in progressive cholestasis and hepatic failure (5).
- Athman, R. et al. (2002) Am. J. Physiol. Gastrointest. Liver Physiol. 283, G496-502.
- Kumar, N. et al. (2004) J. Biol. Chem. 279, 45036-45046.
- Tomar, A. et al. (2004) Mol. Biol. Cell 15, 4807-4817.
- Suh, N. et al. (2005) Mod. Pathol. 18, 1217-1222.
- Phillips, M.J. et al. (2003) Lancet 362, 1112-1119.
Application References
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
