Product Pathways - NF-kB Signaling
IKKβ (2C8) Rabbit mAb #2370
|2370S||100 µl (10 western blots)||---||In Stock||---|
|2370||carrier free and custom formulation / quantity||email request|
Already purchased this product? Write a Review.
|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||87||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Specificity / Sensitivity
IKKβ (2C8) Rabbit mAb detects endogenous levels of total IKKβ protein. The antibody does not cross-react with IKKα or IKKγ.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues at the carboxy terminus of human IKKβ protein.
The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins (1-3). Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit (8,9). Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes, resulting in kinase activation (10-13).
- Baeuerle, P.A. and Baltimore, D. (1988) Science 242, 540-6.
- Beg, A.A. and Baldwin, A.S. (1993) Genes Dev 7, 2064-70.
- Finco, T.S. et al. (1994) Proc Natl Acad Sci USA 91, 11884-8.
- Brown, K. et al. (1995) Science 267, 1485-8.
- Brockman, J.A. et al. (1995) Mol Cell Biol 15, 2809-18.
- Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
- Chen, Z.J. et al. (1996) Cell 84, 853-62.
- Zandi, E. et al. (1997) Cell 91, 243-52.
- Karin, M. (1999) Oncogene 18, 6867-74.
- DiDonato, J.A. et al. (1997) Nature 388, 548-54.
- Mercurio, F. et al. (1997) Science 278, 860-6.
- Johnson, L.N. et al. (1996) Cell 85, 149-58.
- Delhase, M. et al. (1999) Science 284, 309-13.
- Luedde, T. et al. (2008) Proc Natl Acad Sci U S A 105, 9733-8. Applications: Western Blotting.
- Barisic, S. et al. (2010) Biochem Pharmacol 80, 439-47. Applications: Western Blotting.
- Sarkar, S. et al. (2011) Mol Cell Biol 31, 602-15. Applications: Western Blotting.
- Gardner, A. et al. (2012) Am J Pathol 180, 2293-308. Applications: Western Blotting.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 2078 Phospho-IKKα (Ser176)/IKKβ (Ser177) (C84E11) Rabbit mAb
- 2697 Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb
- 2694 Phospho-IKKα/β (Ser176/180) Antibody II
- 2684 IKKβ Antibody
- 2678 IKKβ (L570) Antibody
- 2682 IKKα Antibody
- 2905 IKKε (D20G4) Rabbit mAb
- 2695 IKKγ (DA10-12) Mouse mAb
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
This antibody is developed, validated, and produced by CST using in part technology under license (granting certain rights including those under U.S. Patents No. 5,675,063 and 7,429,487) from Epitomics, Inc.