Product Pathways - MAPK Signaling

p38α MAP Kinase (7D6) Rabbit mAb #2371

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Applications	Reactivity	MW (kDa)	Source	Isotype
W	H M R Mk	40	Rabbit	IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

p38α MAP Kinase (7D6) Rabbit mAb detects endogenous levels of total p38α MAP kinase protein. This antibody does not cross-react with other p38 MAP kinase isoforms such as β, γ or δ.

Source / Purification

Monoclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) derived from the sequence of human p38α MAP kinase.

Background

p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase which participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAP kinase, p38α, β, γ (also known as ERK6 or SAPK3) and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAP kinase is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharides (LPS), UV light and growth factors (1-5). MKK3, MKK6 and SEK activate p38 MAP kinase by phosphorylation at Thr180 and Tyr182. Activated p38 MAP kinase has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6) and MEF2 (5-8).

1. Rouse, J. et al. (1994) Cell 78, 1027-1037.
2. Han, J. et al. (1994) Science 265, 808-811.
3. Lee, J.C. et al. (1994) Nature 372, 739-746.
4. Freshney, N.W. et al. (1994) Cell 78, 1039-1049.
5. Raingeaud, J. et al. (1995) J. Biol. Chem. 270, 7420-7426.
6. Zervos, A.S. et al. (1995) Proc. Natl. Acad. Sci. USA 92, 10531-10534.
7. Zhao, M. et al. (1999) Mol. Cell. Biol. 19, 21-30.
8. Yang, S.H. et al. (1999) Mol. Cell. Biol. 19, 4028-4038.

Application References

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Companion Products

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• 7071 Phototope^®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide
• 9219 Immobilized Phospho-p38 MAPK (Thr180/Tyr182) Mouse mAb
• 2307 p38γ MAP Kinase Antibody
• 2308 p38δ MAP Kinase (10A8) Rabbit mAb
• 9210 PhosphoPlus® p38 MAP Kinase (Thr180/Tyr182) Antibody Kit

Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.