Product Pathways - Complementary Reagents
β-Gal (14B7) Mouse mAb #2372
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| W IP IF-IC F | All | Transfected Only | Mouse IgG1 |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.
Protocols
Specificity / Sensitivity
Beta-Gal 14B7 Mouse mAb detects transfected beta-galactosidase fusion proteins.
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant beta-galactosidase protein.
Western Blotting
Western blot analysis of cells expressing beta-galactosidase (lane 1) or cells without transfection (lane 2), using Beta-Gal (14B7) Mouse mAb.
IP
Immunoprecipitation of cell extracts overexpressing beta-galactosidase (lane 1) or cells without transfection (lane 2), using Beta-Gal (14B7) Mouse mAb, followed by Western blot analysis using the same antibody.
Flow Cytometry
Flow cytometric analysis of COS cells, untransfected (blue) or transfected with β-galactosidase (green), using β-Gal (14B7) Mouse mAb.
IF-IC
Immunocytochemical staining of cells expressing b-galactosidase (left) or cells without transfection (right), using b-Gal 14B7 Mouse mAb.
Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.
Eukaryotic genes can be cloned into E. coli beta-galactosidase (lacZ) gene (1-3). This results in the expression of a fusion protein containing the desired protein with a beta-gal tag. An antibody to this tag can then be used to isolate the fusion protein or detect its expression within cells or tissues.
- Draber, P. et al. (1992) Hybridoma 11, 385-390.
- Hayashibe, K. et al. (1990) J. Immunoassay 11, 89-95.
- Shapiro, S. and Kimmel, B. (1987) J. Immunol. Methods 97, 275-279.
Application References
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
