β-Gal (14B7) Mouse mAb #2372
|2372S||100 µl (10 western blots)||---||In Stock||---|
|2372||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Transfected Only||Mouse IgG1|
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Specificity / Sensitivity
Beta-Gal 14B7 Mouse mAb detects transfected beta-galactosidase fusion proteins.
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant beta-galactosidase protein.
Western blot analysis of cells expressing beta-galactosidase (lane 1) or cells without transfection (lane 2), using Beta-Gal (14B7) Mouse mAb.
Immunoprecipitation of cell extracts overexpressing beta-galactosidase (lane 1) or cells without transfection (lane 2), using Beta-Gal (14B7) Mouse mAb, followed by Western blot analysis using the same antibody.
Flow cytometric analysis of COS cells, untransfected (blue) or transfected with β-galactosidase (green), using β-Gal (14B7) Mouse mAb.
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.
Eukaryotic genes can be cloned into E. coli beta-galactosidase (lacZ) gene (1-3). This results in the expression of a fusion protein containing the desired protein with a beta-gal tag. An antibody to this tag can then be used to isolate the fusion protein or detect its expression within cells or tissues.
- Draber, P. et al. (1992) Hybridoma 11, 385-390.
- Hayashibe, K. et al. (1990) J. Immunoassay 11, 89-95.
- Shapiro, S. and Kimmel, B. (1987) J. Immunol. Methods 97, 275-279.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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