Product Pathways - Lymphocyte Signaling
Itk (2F12) Mouse mAb #2380
PhosphoSitePlus® protein, site, and accession data: ITK
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP | H (M) (R) (Mk) | Endogenous | 72 | Mouse IgG1 |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
Itk (2F12) Mouse mAB detects endogenouse levels of total Itk protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with an amino-terminal peptide of Itk.
Western Blotting
Western blot analysis of extracts from Jurkat, BaF3, THP-1, RAW and Raji cells, using Itk (2F12) Mouse mAb (upper) and beta-Actin Antibody (#4967) (lower).
Western Blotting
Western blot analysis of extracts from Jurkat and HuT 78 cells, using Itk (2F12) Mouse mAb.
Background
Interleukin-2 inducible T-cell kinase (Itk, Emt or Tsk) is a member of the non-receptor protein tyrosine kinases. Family members of Itk include Tec, Btk, Rlk and Bmx and are all defined by a common structure: an amino-terminal PH domain, a Tec-homology domain and a SH3 and SH2 domain followed by a carboxy-terminal kinase domain (1). Tec, Rlk and Itk are expressed in T cells and activated in response to T cell receptor (TCR) engagement. Data demonstrate that Itk functions in signal transduction downstream of TCR and activates PLCgamma1 and Erk. Lck directly activates Itk through phosphorylation in the conserved activation loop at Tyr511, and furthermore, Itk is autophosphorylated in the SH3 domain at Tyr180. Itk-Y180F is still capable of phosphorylating PLCgamma1 in contrast to Itk-Y511F, which has lost that function (2-3). Itk -/- mice show reduced lung inflammation, eosinophil infiltration and mucous production in response to allergic asthma induction. Thus, Itk could become a desirable target for anti-asthmatic treatments (4).
- Schwartzberg, P.L. and Finkelstein, L.D. (2005) Nat Rev Immunol. 5, 284-295.
- Heyeck, S. D. et al. (1997) J Biol Chem. 272, 25401-25408.
- Wilcox, H.M. and Berg, L.J. (2003) J Biol Chem. 278, 37112-37121.
- Mueller, C. and August, A. (2003) J Immunol. 170, 5056-5063.
Application References
- Heyeck, S. D. et al. (1997) Lck phosphorylates the activation loop tyrosine of the Itk kinase domain and activates Itk kinase activity. J Biol Chem. 272, 25401-25408. Applications: IP Western Blotting
- Wilcox, H.M. and Berg, L.J. (2003) Itk phosphorylation sites are required for functional activity in primary T cells. J Biol Chem. 278, 37112-37121. Applications: IP Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 2751 Phospho-Lck (Tyr505) Antibody
- 2752 Lck Antibody
- 2822 PLCγ1 Antibody
- 3537 Phospho-Btk (Ser180) (3D3) Mouse mAb
- 4987 Tec Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
