Product Pathways - Adhesion
CEA/CD66e (CB30) Mouse mAb #2383
| Applications | Reactivity | Sensitivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|---|
| W IHC-P IF-IC F | H | Endogenous | 180-200 | Mouse | IgG1 |
Applications Key:
W=Western Blotting
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
CEA/CD66e (CB30) Mouse mAb detects endogenous levels of CEA protein.
Source / Purification
Monoclonal antibody (Isotype: IgG1) is produced by immunizing mice with human CEA.
Western Blotting
Western blot analysis of extracts from HT29 and MCF7 cells, using CEA/CD66e (CB30) Mouse mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using CEA/CD66e (CB30) Mouse mAb.
Flow Cytometry
Flow cytometric analysis of HT-29 cells, using CEA/CD66e (CB30) Mouse mAb (blue) compared to a nonspecific negative control antibody (red).
IF-IC
Immunofluorescent analysis of MCF7 cells, using CEA/CD66e (CB30) Mouse mAb.
Background
Carcinoembryonic antigen (CEA), also known as CD66e or CEACAM5, is a 180-200 kDa cell surface glycoprotein whose expression is elevated in intestinal carcinomas and other tumors. CEA mediates cell adhesion, though little more is known about its biological activity. Expression of CEA is correlated with tumerogenicity (1), and it has been shown to play a role in cell migration, adhesion and invasion in culture cells, as well as in metastasis in vivo (2).
- Baczynska, D. et al. (2003) Cell Mol Biol Lett 8, 471-86.
- Blumenthal, R.D. et al. (2005) Cancer Res 65, 8809-17.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
