Cell Signaling Technology

Product Pathways - Wnt / Hedgehog / Notch

Wnt5a Antibody #2392

Applications Reactivity Sensitivity MW (kDa) Source
W M (H) Transfected Only 45 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Wnt5a Antibody detects transfected Wnt5a protein in L cells.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu270 of human Wnt5a. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of total cell extracts from L cells overexpressing mouse Wnt5a cells compared to parental L cells, using Wnt5a Antibody.

Background

The Wnt family includes several secreted glycoproteins that play important roles in animal development (1). There are 19 Wnt genes in the human genome that encode functionally distinct Wnt proteins (2). Wnt members bind to the Frizzled family of seven-pass transmembrane proteins and activate several signaling pathways (3). The canonical Wnt/β-catenin pathway also requires a coreceptor from the low-density lipoprotein receptor family (4). Aberrant activation of Wnt signaling pathways is involved in several types of cancers (5).

Wnt-5a has been shown to signal through the canonical Wnt pathways as well as through non-canonical pathways and is up-regulated in various types of human cancers (6-8). In melanoma, Wnt5a is thought to directly affect cell motility and metastasis (9).

  1. Cadigan, K.M. and Nusse, R. (1997) Genes Dev. 11, 3286-3305.
  2. Moon, R.T. et al. (1997) Trends Genet. 13, 157-162.
  3. Kohn, A.D. and Moon, R.T. (2004) Cell Calcium 38, 439-446.
  4. Logan, C.Y. and Nusse, R. (2004) Annu. Rev. Cell Dev. Biol. 20, 781-810.
  5. Giles, R.H. et al. (2003) Biochim. Biophys. Acta. 1653, 1-24.
  6. Mikels, A.J. and Nusse, R. (2006) PLoS Biol. 4, e115.
  7. Katoh, M. and Katoh, M. (2007) Int J Mol Med 19, 273-278.
  8. Katoh, M. (2005) Oncol. Rep. 14, 1583-1588.
  9. Weeraratna, A.T. et al. (2002) Cancer Cell 1, 279-288.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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