Cell Signaling Technology

Product Pathways - Ca / cAMP / Lipid Signaling

PDE5 Antibody #2395

Applications Reactivity Sensitivity MW (kDa) Source
W H M Endogenous 100 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

PDE5 Antibody detects endogenous levels of total PDE5 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminal sequence of human PDE5. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extract from LN-18 cells, using PDE5 Antibody.

Background

The superfamily of phosphodiesterases (PDEs) catalyses the hydrolysis of 3',5'-cyclic nucleotides into the corresponding nucleotide 5'-monophosphates. PDE5 is a cGMP-specific enzyme that contains two cGMP binding sites in its amino-terminal regulatory domain (1). Elevation of cGMP levels causes activation of PKG, which phosphorylates PDE5 at Ser102 (2). Phosphorylation of PDE5 stimulates its enzymatic activity and enhances cGMP binding affinity in the regulatory domain, leading to a decrease in intracellular cGMP levels (3,4).

  1. Corbin, J.D. and Francis, S.H. (1999) J. Biol. Chem. 274, 13729-13732.
  2. Thomas, L.K. et al. (1990) J. Biol. Chem. 265, 14971-14978.
  3. Rybalkin, S.D. et al. (2002) J. Biol. Chem. 277, 3310-3317.
  4. Francis, S.H. et al. (2002) J. Biol. Chem. 277, 47581-47587.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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