Product Pathways - Companion Products
MBP (8G1) Mouse mAb #2396
PhosphoSitePlus® protein, site, and accession data: MBP
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| W IP IF-IC | All | Transfected Only | Mouse IgG1 |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.
Protocols
Specificity / Sensitivity
MBP (8G1) Mouse mAb detects recombinant proteins fused to maltose binding protein (MBP).
Source / Purification
Monoclonal antibody is produced by immunizing animals with an MBP fusion protein.
Western Blotting
Western blot analysis of Sf9 insect cells expressing MBP-Akt fusion protein (lane 1) or cells without transfection (lane 2), using MBP (8G1) Mouse mAb.
IP
Immunoprecipitation of Sf9 insect cell extract overexpressing MBP-Akt fusion protein (lane 1) or cells without transfection (lane 2), using MBP (8G1) Mouse mAb, then Western analysis using the same antibody.
IF-IC
Immunocytochemical staining of Sf9 insect cells expressing MBP fusion protein (left) or cells without transfection (right), using MBP (8G1) Mouse mAb.
Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.
One commonly used system is the maltose-binding protein (MBP) (1). In this system, the target gene is inserted in the appropriate vector and expressed. Following purification using an amylose column, the MBP-target protein complex can be eluted from the column with maltose and cleaved with a specific protease to yield the purified target protein (2). An antibody to MBP can also be used to isolate or detect expression of the protein.
- di Guan, C. et al. (1988) Gene 67, 21-30.
- Kellermann, O.K. and Ferenci, T. (1982) Methods Enzymol. 90, 459-463.
Application References
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For Research Use Only. Not For Use In Diagnostic Procedures.
