Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Vav3 Antibody #2398

Applications Reactivity Sensitivity MW (kDa) Source
W H Endogenous 98 kDa Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Vav3 Antibody detects endogenous levels of total Vav3 protein. The antibody does not cross-react with endogenous levels of other Vav family members.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the central sequence of human Vav3. Antibodies are purified using protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat, MCF-7 and HUVEC cells using Vav3 Antibody.

Background

Vav proteins belong to the Dbl family of guanine nucleotide exchange factors (GEFs) for Rho/Rac small GTPases. The three identified mammalian Vav proteins (Vav1, Vav2 and Vav3) differ in their expression. Vav1 is expressed only in hematopoietic cells and is involved in the formation of the immune synapse. Vav2 and Vav3 are more ubiquitously expressed. Vav proteins contain the Dbl homology domain, which confers GEF activity, as well as protein interaction domains that allow them to function in pathways regulating actin cytoskeleton organization (reviewed in 1). Phosphorylation stimulates the GEF activity of Vav protein towards Rho/Rac (2,3).

  1. Hornstein, I. et al. (2004) Cell. Signal. 16, 1-11.
  2. Crespo, P. et al. (1997) Nature 385, 169-172.
  3. Han, J. et al. (1997) Mol. Cell. Biol. 17, 1346-1353.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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