Product Pathways - Cytoskeletal Signaling
Rap1A/Rap1B (26B4) Rabbit mAb #2399
|2399S||100 µl (10 western blots)||---||In Stock||---|
|2399||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Monkey, Bovine||Endogenous||21||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Specificity / Sensitivity
Rap1 (26B4) Rabbit mAb detects endogenous levels of total Rap1A and Rap1B protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with synthetic peptides corresponding to the carboxy-terminal half of human Rap1A/RAP1B.
Rap1 and Rap2 belong to the Ras subfamily of small GTPases and are activated by a wide variety of stimuli through integrins, receptor tyrosine kinases (RTKs), G-protein coupled receptors (GPCR), death domain associated receptors (DD-R) and ion channels (1,2). Like other small GTPases, Rap activity is stimulated by guanine nucleotide exchange factors (GEF) and inactivated by GTPase activating proteins (GAP). A wide variety of Rap GEFs have been identified: C3G connects Rap1 with RTKs through adaptor proteins such as Crk, Epacs (or cAMP-GEFs) transmit signals from cAMP, and CD-GEFs (or CalDAG-GEFs) convey signals from either or both Ca2+ and DAG (1). Rap1 primarily regulates multiple integrin-dependent processes such as morphogenesis, cell-cell adhesion, hematopoiesis, leukocyte migration and tumor invasion (1,2). Rap1 may also regulate proliferation, differentiation and survival through downstream effectors including B-Raf, PI3K, RalGEF and phospholipases (PLCs) (1-4). Rap1 and Rap2 are not fuctionally redundant as they perform overlapping but distinct functions (5). Recent research indicates that Rap2 regulates Dsh subcellular localization and is required for Wnt signaling in early development (6).
- Bos, J. et al. (2001) Nat. Rev. Mol. Cell Biol. 2, 369-377.
- Caron, E. (2003) J. Cell Sci. 116, 435-440.
- Song, C. et al. (2002) Oncogene 21, 8105-8113.
- Rong, R. et al. (2003) J. Biol. Chem. 278, 52497-52503.
- Taira, K. et al. (2004) J. Biol. Chem. 279, 49488-49496.
- Choi, S. and Han, J. (2005) EMBO J. 24, 985-996.
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
This antibody is developed, validated, and produced by CST using in part technology under license (granting certain rights including those under U.S. Patents No. 5,675,063 and 7,429,487) from Epitomics, Inc.