Product Pathways - Cytoskeletal Signaling
Clathrin Heavy Chain (P1663) Antibody #2410
Have you tried your application using our XP® monoclonal antibodies? Try product: 4796
PhosphoSitePlus® protein, site, and accession data: CLTC
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IF-IC | H M R Mk B | Endogenous | 190 | Rabbit |
Applications Key:
W=Western Blotting
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
Clathrin Heavy Chain (P1663) Antibody detects endogenous levels of total Clathrin protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro1663 of human Clathrin. Antibodies are purified by protein A and peptide affinity chromatography
Western Blotting
Western blot analysis of extracts from SHSY5Y, Neuro2A, PC12 and COS cells, using Clathrin Heavy Chain (P1663) Antibody.
IF-IC
Confocal immunofluorescent analysis of HeLa cells labeled with Clathrin Heavy Chain (P1663) Antibody (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Background
Clathrin-coated vesicles provide for the intracellular transport of cargo proteins following endocytosis and during multiple vesicle trafficking pathways. Vesicles form at specialized areas of the cell membrane where clathrin and associated proteins form clathrin-coated pits. Invagination of these cell membrane-associated pits internalizes proteins and forms an intracellular clathrin-coated vesicle (1,2). Clathrin is the most abundant protein in these vesicles and is present as a basic assembly unit called a triskelion. Each clathrin triskelion is composed of three clathrin heavy chains and three clathrin light chains. Clathrin heavy chain proteins are composed of several functional domains, including a carboxy-terminal region that permits interaction with other heavy chain proteins within a triskelion, and a globular amino-terminal region that associates with other vesicle proteins (2). Adaptor proteins, such as AP2, epsin and EPS15, are responsible for the recruitment of vesicle proteins to sites of pit formation and the assembly of the clathrin-coated vesicle. Following vesicle invagination, the GTPase dynamin constricts the neck of the nascent vesicle to complete formation of the free, cytosolic vesicle (3,4).
- Rodriguez-Boulan, E. et al. (2005) Nat. Rev. Mol. Cell Biol. 6, 233-247.
- Mousavi, S.A. et al. (2004) Biochem. J. 377, 1-16.
- Rappoport, J.Z. et al. (2004) Traffic 5, 327-237.
- Brett, T.J. and Traub, L.M. (2006) Curr. Opin. Cell Biol. 18, 395-406.
Application References
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
