Product Pathways - Cytoskeletal Signaling
EEA1 Antibody #2411
PhosphoSitePlus® protein, site, and accession data: EEA1
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP IF-IC | H M R Mk | Endogenous | 170 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
EEA1 Antibody detects endogenous levels of total EEA1 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly14 of human EEA1 protein. Antibodies are purified by peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from various cell types using EEA1 Antibody.
IF-IC
Confocal immunofluorescent analysis of NIH/3T3 cells using EEA1 Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Background
EEA1 is an early endosomal marker and a Rab5 effector protein essential for early endosomal membrane fusion and trafficking (1-2). The carboxy terminus of EEA1 contains a FYVE domain which binds to phosphatidylinositol-3-phosphate (PtdIns(3)P), targeting EEA1 to early endosomes (3). The stable association of EEA1 with the endosomal membrane is regulated by PI3 kinase, Rab5 and calcium/calmodulin (4-6). Once on the membrane, EEA1 interacts with Rab5, NSF and syntaxin 13 to promote early endosomal membrane docking and fusion (7).
- Mu, F.T. et al. (1995) J. Biol. Chem. 270, 13503-13511.
- Christoforidis, S. et al. (1999) Nature 397, 621-625.
- Gaullier, J.M. et al. (1998) Nature 394, 432-433.
- Patki, V. et al. (1997) Proc. Natl. Acad. Sci. USA 94, 7326-7330.
- Lawe, D.C. et al. (2003) Mol. Biol. Cell 14, 2935-2945.
- Simonsen, A. et al. (1998) Nature 394, 494-498.
- McBride, H.M. et al. (1999) Cell 98, 377-386.
Application References
- Fort, A.G. et al. (2011) J Biol Chem 286, 22875-85. Applications: IC-IF
- Lowther, K.M. et al. (2011) Reproduction 141, 737-47. Applications: IF-IC (In Cells) Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7075 Anti-biotin, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 2143 Rab5 Antibody
- 2145 NSF Antibody
- 2410 Clathrin Heavy Chain (P1663) Antibody
- 3288 EEA1 (C45B10) Rabbit mAb
For Research Use Only. Not For Use In Diagnostic Procedures.
