Product Pathways - Cytoskeletal Signaling
EEA1 Antibody #2411
|W IP IF-IC||H M R Mk||Endogenous||170||Rabbit|
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
EEA1 Antibody detects endogenous levels of total EEA1 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly14 of human EEA1 protein. Antibodies are purified by peptide affinity chromatography.
EEA1 is an early endosomal marker and a Rab5 effector protein essential for early endosomal membrane fusion and trafficking (1-2). The carboxy terminus of EEA1 contains a FYVE domain which binds to phosphatidylinositol-3-phosphate (PtdIns(3)P), targeting EEA1 to early endosomes (3). The stable association of EEA1 with the endosomal membrane is regulated by PI3 kinase, Rab5 and calcium/calmodulin (4-6). Once on the membrane, EEA1 interacts with Rab5, NSF and syntaxin 13 to promote early endosomal membrane docking and fusion (7).
- Mu, F.T. et al. (1995) J. Biol. Chem. 270, 13503-13511.
- Christoforidis, S. et al. (1999) Nature 397, 621-625.
- Gaullier, J.M. et al. (1998) Nature 394, 432-433.
- Patki, V. et al. (1997) Proc. Natl. Acad. Sci. USA 94, 7326-7330.
- Lawe, D.C. et al. (2003) Mol. Biol. Cell 14, 2935-2945.
- Simonsen, A. et al. (1998) Nature 394, 494-498.
- McBride, H.M. et al. (1999) Cell 98, 377-386.
- Fort, A.G. et al. (2011) J Biol Chem 286, 22875-85. Applications: IC-IF
- Lowther, K.M. et al. (2011) Reproduction 141, 737-47. Applications: IF-IC (In Cells) Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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For Research Use Only. Not For Use In Diagnostic Procedures.