Cell Signaling Technology

Product Pathways - Protein Stability

PA28γ Antibody #2412

Applications Reactivity Sensitivity MW (kDa) Source
W IP IHC-P IF-IC H M R Mk Endogenous 30 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

PA28γ Antibody detects endogenous levels of total PA28γ.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp16 of human PA2γ. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from MCF-7, Neuro2A, PC12 and COS cells using PA28γ Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using PA28γ Antibody in the presence of control peptide (left) or antigen-specific peptide (right).

IF-IC

IF-IC

Confocal immunofluorescent analysis of MCF-7 cells using PA28γ Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).


Background

The 20S proteasome is the major proteolytic enzyme complex involved in intracellular protein degradation. PA700, PA28, and PA200 are three major protein complexes that function as activators of the 20S proteasome. There are three evolutionarily conserved subunits of PA28: PA28α (PSME1), PA28β (PSME2), and PA28γ (PSME3) (1,2). PA28α and PA28β form a heteroheptameric complex and function by binding to the 20S complex at its opening site(s). The PA28α/β complex is present throughout the cell and participates in MHC class I antigen presentation by promoting the generation of antigenic peptides from foreign proteins (2). PA28γ exists in the form of a homoheptamer and is mainly located in the nucleus. The PA28γ complex exerts its function by binding and guiding specific nuclear target proteins to the 20S proteasome for further degradation (3,4).

  1. Dahlmann, B. (2005) Essays Biochem. 41, 31-48.
  2. Rechsteiner, M. and Hill, C.P. (2005) Trends Cell Biol. 15, 27-33.
  3. Zhou, P. (2006) Cell 124, 256-257.
  4. Li, X. et al. (2006) Cell 124, 381-392.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Products