Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Rab11a Antibody #2413

Applications Reactivity Sensitivity MW (kDa) Source
W IF-IC H M R Mk Endogenous 25 Rabbit

Applications Key:  W=Western Blotting  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Rab11a Antibody detects endogenous levels of total Rab11a protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg184 of human Rab11a protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Rab11a Antibody.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using Rab11a Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Background

Rab11a, Rab11b and Rab25 are members of the Rab11 family of small Ras-like GTPases. Rab11 (isoforms Rab11a and Rab11b) functions as a key regulator in the recycling of perinuclear, plasma membrane and Golgi compartment endosomes (1,2). Despite some overlap, distinct differences exist between Rab11a and Rab11b in both their cellular distribution and functional roles. Rab11a is ubiquitously expressed while Rab11b is found mainly in the heart and brain (3,4). Like other Rab proteins, Rab11 exerts its function via interactions with Rab11 family interacting proteins (FIPs). While there are three distinct classes of FIPs, all appear to share a conserved carboxy-terminal Rab-binding domain that allows Rab-FIP protein interaction. When bound together, these proteins are thought to regulate membrane-associated protein sorting (5,6).

  1. Ullrich, O. et al. (1996) J. Cell Biol. 135, 913-924.
  2. Chen, W. et al. (1998) Mol. Biol. Cell 9, 3241-3257.
  3. Lapierre, L.A. et al. (2003) Exp. Cell Res. 290, 322-331.
  4. Khvotchev, M.V. et al. (2003) J. Neurosci. 23, 10531-10539.
  5. Junutula, J.R. et al. (2004) J. Biol. Chem. 279, 33430-33437.
  6. Hales, C.M. et al. (2001) J. Biol. Chem. 276, 39067-39075.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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